ArticlePloS one2017
Downregulation of microRNA-145 may contribute to liver fibrosis in biliary atresia by targeting ADD3.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 37 citations in OpenAlex.
- The correlation between rs2501577 gene polymorphism and biliary atresia: a systematic review and meta-analysis.Pediatric surgery international · 2023Pooled it
- Elevated Epithelial Splicing Regulatory Protein 1 Expression in Biliary Atresia Indicates Its Potential as a Molecular Marker.Biomolecules · 2025Article
- Melatonin-pretreated mesenchymal stem cell-derived exosomes alleviate cavernous fibrosis in a rat model of nerve injury-induced erectile dysfunction via miR-145-5p/TGF-β/Smad axis.Stem cell research & therapy · 2025Article
- Genetic background and biliary atresia.World journal of pediatric surgery · 2025Review
- Multiple susceptibility enhancer variants increasingFrontiers in genetics · 2025Article
- Molecular Mechanisms of Fibrosis in Cholestatic Liver Diseases and Regenerative Medicine-Based Therapies.Cells · 2024Review
- Role of the circular RNA regulatory network in the pathogenesis of biliary atresia.Experimental and therapeutic medicine · 2024Article
- Comparison of Mir122 expression in children with biliary atresia and healthy group.Molecular biology research communications · 2024Article
- Long non-coding RNA growth arrest-specific 5 inhibits liver fibrogenesis in biliary atresia by interacting with microRNA-222 and repressing IGF1/AKT signaling.Translational pediatrics · 2023Article
- Contribution ofInternational journal of molecular sciences · 2023Article
- Genetic Contributions to Biliary Atresia: A Developmental Cholangiopathy.Seminars in liver disease · 2023Review
- Role of long non-coding RNA-adducin 3 antisense RNA1 in liver fibrosis of biliary atresia.Bioengineered · 2022Article
- The role of let-7b in the inhibition of hepatic stellate cell activation by rSjP40.PLoS neglected tropical diseases · 2021Article
- Inhibition of MicroRNA 6937 Delays Photoreceptor and Vision Loss in a Mouse Model of Retinitis Pigmentosa.Pharmaceutics · 2020Article
- Downregulation of miR-152 contributes to the progression of liver fibrosis via targeting Gli3Experimental and therapeutic medicine · 2019Article
- let-7a suppresses liver fibrosis via TGFβ/SMAD signaling transduction pathway.Experimental and therapeutic medicine · 2019Article
- Application of Weighted Gene Co-Expression Network Analysis to Explore the Key Genes in Alzheimer's Disease.Journal of Alzheimer's disease : JAD · 2018Article
- A Review on Adducin from Functional to Pathological Mechanisms: Future Direction in Cancer.BioMed research international · 2018Review
Corrections and comments
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Authors and funding
12 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
objectivesBiliary atresia (BA) is a pediatric liver disease characterized by fibro-obliteration and obstruction of the extrahepatic biliary system, that invariably leads to cirrhosis and even death, if left untreated for extended time. However, its pathology and etiology still remained unknown. In this study, we tested the expression of adducin 3 (ADD3), the gene identified as a susceptibility gene in BA by GWAS, and uncovered its upstream regulatory microRNA in the pathogenesis of BA.
methodsIn this study, 14 infants with BA and 14 infants with choledochal cyst (CC) were enrolled as experimental group and control group, respectively. ADD3 and microRNA-145 (miR-145) expression profiles in liver tissues of BA and CC were determined using qPCR. Luciferase reporter assay was performed to verify the direct interaction between miR-145-5p and ADD3 3' Untranslated Regions (3'UTR). The Lentiviral vectors containing miR-145, miR-145-3p inhibitor, miR-145-5p inhibitor, empty vector were transfected into human hepatic stellate cell line (LX-2) to determine the functional effect of miR-145 on ADD3 expression at both mRNA and protein level.
resultsMiR-145 was shown to be down-regulated in liver tissues of infants with BA compared to CC (p = 0.0267). ADD3, verified as a target of miR-145-5p, was shown to be overexpressed in infants with BA at the mRNA level (p = 0.0118). Transfection of lentiviruses containing miR-145 into LX-2 cells decreased the expression of ADD3 at both mRNA and protein level compared to negative control group, and suppressed the expression of p-Akt at protein level.
conclusionsOur study has shown that overexpressed ADD3 and downregulated miR-145 were detected in BA liver tissues. MiR-145-5p was confirmed to target ADD3 by luciferase reporter assay. The downregulation of miR-145 may contribute to liver fibrosis in BA by upregulating the expression of ADD3.
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Registered trials
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