Evidence map›Paper›PMID 28900438›Full record

ArticleFrontiers in plant science2017

Chloroplasts Isolation from

Miao Yang, Jun-Peng Jiang, Xi Xie, Ya-Dong Chu, Yan Fan, Xu-Peng Cao, Song Xue, Zhan-You Chi

Open access · goldAbstract read
In one paragraph

Article in Frontiers in plant science, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.7field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 14 citations in OpenAlex.

  1. Article
  2. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 6 institutions in 1 country.

Miao YangMarine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of SciencesDalian, China.
Jun-Peng JiangMarine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of SciencesDalian, China.
Xi XieLiaoning Ocean and Fisheries Science Research InstituteDalian, China.
Ya-Dong ChuMarine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of SciencesDalian, China.
Yan FanMarine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of SciencesDalian, China.
Xu-Peng CaoMarine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of SciencesDalian, China.
Song XueMarine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of SciencesDalian, China.
Zhan-You ChiSchool of Life Sciences and Biotechnology, Dalian University of TechnologyDalian, China.
Dalian Institute of Chemical Physics · CNChinese Academy of Sciences · CNDalian University · CNDalian University of Technology · CNLiaoning Ocean and Fisheries Research Institute · CNUniversity of Chinese Academy of Sciences · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Triacylglycerols are produced in abundance through chloroplast and endoplasmic reticulum pathways in some microalgae exposed to stress, though the relative contribution of either pathway remains elusive. Characterization of these pathways requires isolation of the organelles. In this study, an efficient and reproducible approach, including homogenous batch cultures of nitrogen-deprived algal cells in photobioreactors, gentle cell disruption using a simple custom-made disruptor with mechanical shear force, optimized differential centrifugation and Percoll density gradient centrifugation, was developed to isolate chloroplasts from

Indexed as

Chlamydomonaschloroplast isolationfatty acid biomarkersnitrogen stresstriacylglycerol

Identifiers

PMID28900438
PMCPMC5581827
OpenAlexW2752258018

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.