ArticleScientific reports2017
Partitioning the roles of CYP6G1 and gut microbes in the metabolism of the insecticide imidacloprid in Drosophila melanogaster.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Diving Deeper Into Mechanisms of Acrylamide-Induced Toxicity: RNA Sequencing Reveals Transcriptomic Alteration and Retrotransposon Expression in Drosophila melanogaster.Environmental toxicology · 2026Article
- Insecticide Resistance Alters Oviposition Preference inEcology and evolution · 2026Article
- Mechanisms and Genetic Drivers of Resistance of Insect Pests to Insecticides and Approaches to Its Control.Toxics · 2025Review
- Review
- CRISPR/Cas Technology in Insect Insecticide Resistance.Insects · 2025Review
- Overexpression of CYP6CY1 is Involved in Imidacloprid Resistance in Sitobion miscanthi (Takahashi) (Homoptera: Aphidae).Neotropical entomology · 2025Article
- Sesamin Exerts an Antioxidative Effect by Activating the Nrf2 Transcription Factor in the Glial Cells of the Central Nervous System inAntioxidants (Basel, Switzerland) · 2024Article
- Microbial Symbiont-Based Detoxification of Different Phytotoxins and Synthetic Toxic Chemicals in Insect Pests and Pollinators.Journal of xenobiotics · 2024Review
- Review
- Gut Bacteria Promote Phosphine Susceptibility ofInsects · 2023Article
- RNA interference in cytochrome P450 monooxygenase (CYP) gene results in reduced insecticide resistance inFrontiers in physiology · 2023Article
- High nitrogen in maize enriches gut microbiota conferring insecticide tolerance in lepidopteran pestiScience · 2022Article
- Contribution of insect gut microbiota and their associated enzymes in insect physiology and biodegradation of pesticides.Frontiers in microbiology · 2022Review
- Role of Insect Gut Microbiota in Pesticide Degradation: A Review.Frontiers in microbiology · 2022Review
- Microbial Technologies Employed for Biodegradation of Neonicotinoids in the Agroecosystem.Frontiers in microbiology · 2021Review
- Novel Experimental Methods for the Investigation of Hermetia illucens (Diptera: Stratiomyidae) Larvae.Journal of insect science (Online) · 2020Article
- Oligotrophic bacterium Hymenobacter latericoloratus CGMCC 16346 degrades the neonicotinoid imidacloprid in surface water.AMB Express · 2020Article
- Insights Into the Microbial Degradation and Biochemical Mechanisms of Neonicotinoids.Frontiers in microbiology · 2020Review
- Imidacloprid Decreases Honey Bee Survival Rates but Does Not Affect the Gut Microbiome.Applied and environmental microbiology · 2018Article
- Multiple P450s and Variation in Neuronal Genes Underpins the Response to the Insecticide Imidacloprid in a Population of Drosophila melanogaster.Scientific reports · 2017Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Resistance to insecticides through enhanced metabolism is a worldwide problem. The Cyp6g1 gene of the vinegar fly, Drosophila melanogaster, is a paradigm for the study of metabolic resistance. Constitutive overexpression of this gene confers resistance to several classes of insecticides, including the neonicotinoid imidacloprid (IMI). The metabolism of IMI in this species has been previously shown to yield oxidative and nitro-reduced metabolites. While levels of the oxidative metabolites are correlated with CYP6G1 expression, nitro-reduced metabolites are not, raising the question of how these metabolites are produced. Some IMI metabolites are known to be toxic, making their fate within the insect a second question of interest. These questions have been addressed by coupling the genetic tools of gene overexpression and CRISPR gene knock-out with the mass spectrometric technique, the Twin-Ion Method (TIM). Analysing axenic larvae indicated that microbes living within D. melanogaster are largely responsible for the production of the nitro-reduced metabolites. Knock-out of Cyp6g1 revealed functional redundancy, with some metabolites produced by CYP6G1 still detected. IMI metabolism was shown to produce toxic products that are not further metabolized but readily excreted, even when produced in the Central Nervous System (CNS), highlighting the significance of transport and excretion in metabolic resistance.
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