ArticleScientific reports2017
Projection Stereolithographic Fabrication of BMP-2 Gene-activated Matrix for Bone Tissue Engineering.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 39 citations in OpenAlex.
- Trends in Photopolymerization 3D Printing for Advanced Drug Delivery Applications.Biomacromolecules · 2025Review
- Mesenchymal stromal cell-laden hydrogels in tissue regeneration: insights from preclinical and clinical research.Frontiers in pharmacology · 2025Review
- Research progress of gene therapy combined with tissue engineering to promote bone regeneration.APL bioengineering · 2024Review
- Resorbable GBR Scaffolds in Oral and Maxillofacial Tissue Engineering: Design, Fabrication, and Applications.Journal of clinical medicine · 2023Review
- Review
- Recapitulation of growth factor-enriched microenvironment via BMP receptor activating hydrogel.Bioactive materials · 2023Article
- Graphene oxide-functionalized nanocomposites promote osteogenesis of human mesenchymal stem cells via enhancement of BMP-SMAD1/5 signaling pathway.Biomaterials · 2021Article
- 3D Printing and Bioprinting to Model Bone Cancer: The Role of Materials and Nanoscale Cues in Directing Cell Behavior.Cancers · 2021Review
- Caveolin-1 mediates soft scaffold-enhanced adipogenesis of human mesenchymal stem cells.Stem cell research & therapy · 2021Article
- Injectable BMP-2 gene-activated scaffold for the repair of cranial bone defect in mice.Stem cells translational medicine · 2020Article
- Burst, Short, and Sustained Vitamin DInternational journal of molecular sciences · 2020Article
- Natural Polymeric Scaffolds in Bone Regeneration.Frontiers in bioengineering and biotechnology · 2020Review
- Integration of biological systems with electronic-mechanical assemblies.Acta biomaterialia · 2019Review
- Efficient in vivo bone formation by BMP-2 engineered human mesenchymal stem cells encapsulated in a projection stereolithographically fabricated hydrogel scaffold.Stem cell research & therapy · 2019Article
- Enhancing chondrogenesis and mechanical strength retention in physiologically relevant hydrogels with incorporation of hyaluronic acid and direct loading of TGF-β.Acta biomaterialia · 2019Article
- An Introduction to 3D Bioprinting: Possibilities, Challenges and Future Aspects.Materials (Basel, Switzerland) · 2018Review
- Enhancement of osteogenesis of rabbit bone marrow derived mesenchymal stem cells by transfection of human BMP-2 and EGFP recombinant adenovirus via Wnt signaling pathway.Experimental and therapeutic medicine · 2018Article
- Additive Manufacturing for Guided Bone Regeneration: A Perspective for Alveolar Ridge Augmentation.International journal of molecular sciences · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Currently, sustained in vivo delivery of active bone morphogenetic protein-2 (BMP-2) protein to responsive target cells, such as bone marrow-derived mesenchymal stem cells (BMSCs), remains challenging. Ex vivo gene transfer method, while efficient, requires additional operation for cell culture and therefore, is not compatible with point-of-care treatment. In this study, two lentiviral gene constructs - (1) Lv-BMP/GFP, containing human BMP-2 and green fluorescent protein (GFP) gene (BMP group); or (2) Lv-GFP, containing GFP gene (GFP group) - were incorporated with human BMSCs into a solution of photocrosslinkable gelatin, which was then subjected to visible light-based projection stereolithographic printing to form a scaffold with desired architectures. Upon in vitro culture, compared to the GFP group, cells from BMP group showed >1,000-fold higher BMP-2 release, and the majority of them stained intensely for alkaline phosphatase activity. Real-time RT-PCR also showed dramatically increased expression of osteogenesis marker genes only in the BMP group. 3.5 months post-implantation into SCID mice, the micro-computed tomography imaging showed detectable mineralized areas only in the BMP group, which was restricted within the scaffolds. Alizarin red staining and immunohistochemistry of GFP and osteocalcin further indicated that the grafted hBMSCs, not host cells, contributed primarily to the newly formed bone.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.