ArticlePloS one2017
Assessment of Listeria monocytogenes virulence in the Galleria mellonella insect larvae model.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- When Small Meets Smaller: Immune Modulation and Virulence Strategies in Insect-Bacteria Interactions.Insects · 2026Review
- Decoding Virulence Mechanisms ofMicroorganisms · 2026Article
- Article
- Listeria monocytogenes: from a saprophytic to a cytosolic virulent pathogen.World journal of microbiology & biotechnology · 2025Review
- Comparative Analysis ofMicroorganisms · 2025Article
- Comparative Analysis of Growth, Survival, and Virulence Characteristics ofMicroorganisms · 2024Article
- Article
- Current methodologies available to evaluate the virulence potential amongFrontiers in microbiology · 2024Review
- Galleria mellonella-intracellular bacteria pathogen infection models: the ins and outs.FEMS microbiology reviews · 2023Review
- On the relationship between Pathogenic Potential and Infective Inoculum.PLoS pathogens · 2022Article
- Ceviche-Natural Preservative: Possibility of Microbiota Survival and Effect onFoods (Basel, Switzerland) · 2022Article
- Involvement of a putative ATP-Binding Cassette (ABC) Involved in manganese transport in virulence of Listeria monocytogenes.PloS one · 2022Article
- Assessment of the Prevalence and Drug Susceptibility ofFoods (Basel, Switzerland) · 2020Article
- Multiple drug resistance ofIranian journal of microbiology · 2020Article
- Virulence assessment of Listeria monocytogenes grown in different foods using a Galleria mellonella model.PloS one · 2020Article
- Effect of Temperatures Used in Food Storage on Duration of Heat Stress Induced Invasiveness ofMicroorganisms · 2019Article
- New Perspectives on Galleria mellonella Larvae as a Host Model Using Riemerella anatipestifer as a Proof of Concept.Infection and immunity · 2019Article
- Simple Evaluation ofFood science of animal resources · 2019Article
- Article
- A parasitological evaluation of edible insects and their role in the transmission of parasitic diseases to humans and animals.PloS one · 2019Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several animal models have been used to understand the molecular basis of the pathogenicity, infectious dose and strain to strain variation of Listeria monocytogenes. The greater wax worm Galleria mellonella, as an alternative model, provides some useful advantages not available with other models and has already been described as suitable for the virulence assessment of various pathogens including L. monocytogenes. The objectives of this study are: 1) confirming the usefulness of this model with a wide panel of Listeria spp. including non-pathogenic L. innocua, L. seeligeri, L. welshimeri and animal pathogen L. ivanovii; 2) assessment of virulence of several isogenic in-frame deletion mutants in virulence and stress related genes of L. monocytogenes and 3) virulence assessment of paired food and clinical isolates of L. monocytogenes from 14 major listeriosis outbreaks occurred worldwide between 1980 and 2015. Larvae injected with different concentrations of Listeria were incubated at 37°C and monitored over seven days for time needed to kill 50% of larvae (LT50) and to determine change of bacterial population in G. mellonella, 2 and 24 hours post-inoculation. Non-pathogenic members of Listeria and L. ivanovii showed significantly (P < 0.05) higher LT50 (lower virulence) than the wild type L. monocytogenes strains. Isogenic mutants of L. monocytogenes with the deletions in prfA, plcA, hly, actA and virR genes, also showed significantly (P < 0.05) higher LT50 than the wild type strain at the inoculum of 106CFU/larva. Food isolates had significantly (P < 0.05) lower virulence than the paired clinical isolates, at all three inoculum concentrations. L. monocytogenes strains related to non-invasive (gastroenteritis) outbreaks of listeriosis showed significantly (P < 0.05) lower virulence than isolates of the same serotype obtained from outbreaks with invasive symptoms. The difference, however, was dose and strain- dependent. No significant differences in virulence were observed among the serotype tested in this study.
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