ArticleThe Journal of cell biology1987
High lateral mobility of endogenous and transfected alkaline phosphatase: a phosphatidylinositol-anchored membrane protein.
Article in The Journal of cell biology, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 123 citations in OpenAlex.
- A Novel Cartesian Plot Analysis for Fixed Monolayers That Relates Cell Phenotype to Transfer of Contents between Fibroblasts and Cancer Cells by Cell-Projection Pumping.International journal of molecular sciences · 2022Article
- Implication of BBM lipid composition and fluidity in mitigated alkaline phosphatase activity in renal cell carcinoma.Molecular and cellular biochemistry · 2012Article
- Ras diffusion is sensitive to plasma membrane viscosity.Biophysical journal · 2005Article
- Kinematics of red cell aspiration by fluorescence-imaged microdeformation.Biophysical journal · 1996Article
- Replacement of the phospholipid-anchor in the contact site A glycoprotein of D. discoideum by a transmembrane region does not impede cell adhesion but reduces residence time on the cell surface.The Journal of cell biology · 1994Article
- Expression of unique sets of GPI-linked proteins by different primary neurons in vitro.The Journal of cell biology · 1992Article
- GP-2/THP gene family encodes self-binding glycosylphosphatidylinositol-anchored proteins in apical secretory compartments of pancreas and kidney.Proceedings of the National Academy of Sciences of the United States of America · 1992Article
- Expression of collagen, osteocalcin, and bone alkaline phosphatase in a mineralizing rat osteoblastic cell culture.Calcified tissue international · 1992Article
- Influence of receptor lateral mobility on adhesion strengthening between membranes containing LFA-3 and CD2.The Journal of cell biology · 1991Article
- Lateral diffusion of membrane-spanning and glycosylphosphatidylinositol-linked proteins: toward establishing rules governing the lateral mobility of membrane proteins.The Journal of cell biology · 1991Article
- Positive and negative immunoselection for enrichment of two classes of osteoprogenitor cells.The Journal of cell biology · 1991Article
- A single gene encodes membrane-bound and free forms of GP-2, the major glycoprotein in pancreatic secretory (zymogen) granule membranes.Proceedings of the National Academy of Sciences of the United States of America · 1991Article
- Emerging functional roles for the glycosyl-phosphatidylinositol membrane protein anchor.The Journal of membrane biology · 1990Review
- Bile duct ligation-induced redistribution of canalicular antigen in rat hepatocyte plasma membranes demonstrated by immunogold quantitation.Histochemistry · 1990Article
- Transcriptional regulation of osteopontin production in rat osteoblast-like cells by parathyroid hormone.The Journal of cell biology · 1989Article
- Modification of proteins with covalent lipids.Progress in lipid research · 1988Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The lateral mobility of alkaline phosphatase (AP) in the plasma membrane of osteoblastic and nonosteoblastic cells was estimated by fluorescence redistribution after photobleaching in embryonic and in tumor cells, in cells that express AP naturally, and in cells transfected with an expression vector containing AP cDNA. The diffusion coefficient (D) and the mobile fraction, estimated from the percent recovery (%R), were found to be cell-type dependent ranging from (0.58 +/- 0.16) X 10(-9) cm2s-1 and 73.3 +/- 10.5 in rat osteosarcoma cells ROS 17/2.8 to (1.77 +/- 0.51) X 10(-9) cm2s-1 and 82.8 +/- 2.5 in rat osteosarcoma cells UMR106. Similar values of D greater than or equal to 10(-9) cm2s-1 with approximately 80% recovery were also found in fetal rat calvaria cells, transfected skin fibroblasts, and transfected AP-negative osteosarcoma cells ROS 25/1. These values of D are many times greater than "typical" values for membrane proteins, coming close to those of membrane lipid in fetal rat calvaria and ROS 17/2.8 cells (D = [4(-5)] X 10(-9) cm2s-1 with 75-80% recovery), estimated with the hexadecanoyl aminofluorescein probe. In all cell types, phosphatidylinositol (PI)-specific phospholipase C released 60-90% of native and transfection-expressed AP, demonstrating that, as in other tissue types, AP in these cells is anchored in the membrane via a linkage to PI. These results indicate that the transfected cells used in this study possess the machinery for AP insertion into the membrane and its binding to PI. The fast AP mobility appears to be an intrinsic property of the way the protein is anchored in the membrane, a conclusion with general implications for the understanding of the slow diffusion of other membrane proteins.
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