ArticleeLife2017
A synthetic biology approach to probing nucleosome symmetry.
Article in eLife, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed.
- Article
- Nucleosomal asymmetry: a novel mechanism to regulate nucleosome function.Biochemical Society transactions · 2024Review
- Rapid reconstitution of ubiquitinated nucleosome using a non-denatured histone octamer ubiquitylation approach.Cell & bioscience · 2024Article
- Histone divergence in trypanosomes results in unique alterations to nucleosome structure.Nucleic acids research · 2023Article
- Native Mass Spectrometry at the Convergence of Structural Biology and Compositional Proteomics.Accounts of chemical research · 2022Article
- A Genetically Encoded Approach for Breaking Chromatin Symmetry.ACS central science · 2022Article
- Spys Make Asymmetric Chromatin.ACS central science · 2022Article
- Janus Bioparticles: Asymmetric Nucleosomes and Their Preparation Using Chemical Biology Approaches.Accounts of chemical research · 2021Review
- Decoding the protein composition of whole nucleosomes with Nuc-MS.Nature methods · 2021Article
- Autoinhibitory elements of the Chd1 remodeler block initiation of twist defects by destabilizing the ATPase motor on the nucleosome.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Novel genetic tools for probing individual H3 molecules in each nucleosome.Current genetics · 2019Review
- An asymmetric centromeric nucleosome.eLife · 2018Article
- NS3 Protein from Rice stripe virus affects the expression of endogenous genes in Nicotiana benthamiana.Virology journal · 2018Article
- Biochemical Analysis of Dimethyl Suberimidate-crosslinked Yeast Nucleosomes.Bio-protocol · 2018Article
Corrections and comments
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Authors and funding
19 authors.
Funding
Abstract
The repeating subunit of chromatin, the nucleosome, includes two copies of each of the four core histones, and several recent studies have reported that asymmetrically-modified nucleosomes occur at regulatory elements in vivo. To probe the mechanisms by which histone modifications are read out, we designed an obligate pair of H3 heterodimers, termed H3X and H3Y, which we extensively validated genetically and biochemically. Comparing the effects of asymmetric histone tail point mutants with those of symmetric double mutants revealed that a single methylated H3K36 per nucleosome was sufficient to silence cryptic transcription in vivo. We also demonstrate the utility of this system for analysis of histone modification crosstalk, using mass spectrometry to separately identify modifications on each H3 molecule within asymmetric nucleosomes. The ability to generate asymmetric nucleosomes in vivo and in vitro provides a powerful and generalizable tool to probe the mechanisms by which H3 tails are read out by effector proteins in the cell.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.