ArticleScientific reports2017
Optogenetic approach for targeted activation of global calcium transients in differentiated C2C12 myotubes.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 28 citations in OpenAlex.
- Physiological and functional characterization for high-throughput optogenetic skeletal muscle exercise assays.Bioengineering & translational medicine · 2026Article
- Optogenetic control of odontoblastic differentiation in dental pulp stem cells (DPSC).Journal of dental sciences · 2026Article
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- Generating fast-twitch myotubes in vitro with an optogenetic-based, quantitative contractility assay.Life science alliance · 2023Article
- Optogenetic Calcium Ion Influx in Myoblasts and Myotubes by Near-Infrared Light Using Upconversion Nanoparticles.ACS applied materials & interfaces · 2023Article
- Optogenetically controlled human functional motor endplate for testing botulinum neurotoxins.Stem cell research & therapy · 2021Article
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- Article
- Towards the clinical translation of optogenetic skeletal muscle stimulation.Pflugers Archiv : European journal of physiology · 2020Review
- hiPSC-Derived Neurons Provide a Robust and Physiologically RelevantFrontiers in pharmacology · 2020Article
- Single-Cell Optogenetic Control of Calcium Signaling with a High-Density Micro-LED Array.iScience · 2019Article
- Spectrally filtered passive Si photodiode array for on-chip fluorescence imaging of intracellular calcium dynamics.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Excitation-contraction coupling in muscle cells is initiated by a restricted membrane depolarization delimited within the neuromuscular junction. This targeted depolarization triggers an action potential that propagates and induces a global cellular calcium response and a consequent contraction. To date, numerous studies have investigated this excitation-calcium response coupling by using different techniques to depolarize muscle cells. However, none of these techniques mimic the temporal and spatial resolution of membrane depolarization observed in the neuromuscular junction. By using optogenetics in C2C12 muscle cells, we developed a technique to study the calcium response following membrane depolarization induced by photostimulations of membrane surface similar or narrower than the neuromuscular junction area. These stimulations coupled to confocal calcium imaging generate a global cellular calcium response that is the consequence of a membrane depolarization propagation. In this context, this technique provides an interesting, contactless and relatively easy way of investigation of calcium increase/release as well as calcium decrease/re-uptake triggered by a propagated membrane depolarization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.