ArticleProceedings of the National Academy of Sciences of the United States of America2017
Engineering a light-activated caspase-3 for precise ablation of neurons in vivo.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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Who cites it
30 citing papers in PubMed, 55 citations in OpenAlex.
- A kinome inhibitor screen implicates adhesion and growth factor signaling in cellular recovery after caspase activation.bioRxiv : the preprint server for biology · 2026Article
- Optogenetic Cancer Therapy Using the Light-Driven Outward Proton Pump Rhodopsin Archaerhodopsin-3 (AR3).Journal of the American Chemical Society · 2025Article
- Optogenetic enzymes: A deep dive into design and impact.Current opinion in structural biology · 2025Review
- Pallidal prototypic neuron and astrocyte activities regulate flexible reward-seeking behaviors.bioRxiv : the preprint server for biology · 2025Article
- Article
- Optogenetic induction of caspase-8 mediated apoptosis by employing Arabidopsis cryptochrome 2.Scientific reports · 2023Article
- External globus pallidus input to the dorsal striatum regulates habitual seeking behavior in male mice.Nature communications · 2023Article
- Axon-axon interactions determine modality-specific wiring and subcellular synaptic specificity in a somatosensory circuit.Development (Cambridge, England) · 2023Article
- Cell survival following direct executioner-caspase activation.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- LOV2-based photoactivatable CaMKII and its application to single synapses: Local Optogenetics.Biophysics and physicobiology · 2023Article
- Precise modulation of embryonic development through optogenetics.Genesis (New York, N.Y. : 2000) · 2022Review
- Spatial and temporal control of expression with light-gated LOV-LexA.G3 (Bethesda, Md.) · 2022Article
- A photo-switchable assay system for dendrite degeneration and repair inProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Optophysiology: Illuminating cell physiology with optogenetics.Physiological reviews · 2022Review
- Optogenetic activators of apoptosis, necroptosis, and pyroptosis.The Journal of cell biology · 2022Article
- CRISPR-Based Transcriptional Activation in Drosophila.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Protein-protein interaction prediction with deep learning: A comprehensive review.Computational and structural biotechnology journal · 2022Review
- Optobiochemistry: Genetically Encoded Control of Protein Activity by Light.Annual review of biochemistry · 2021Review
- Steering Molecular Activity with Optogenetics: Recent Advances and Perspectives.Advanced biology · 2021Review
- Nanoscale programming of cellular and physiological phenotypes: inorganic meets organic programming.NPJ systems biology and applications · 2021Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The circuitry of the brain is characterized by cell heterogeneity, sprawling cellular anatomy, and astonishingly complex patterns of connectivity. Determining how complex neural circuits control behavior is a major challenge that is often approached using surgical, chemical, or transgenic approaches to ablate neurons. However, all these approaches suffer from a lack of precise spatial and temporal control. This drawback would be overcome if cellular ablation could be controlled with light. Cells are naturally and cleanly ablated through apoptosis due to the terminal activation of caspases. Here, we describe the engineering of a light-activated human caspase-3 (Caspase-LOV) by exploiting its natural spring-loaded activation mechanism through rational insertion of the light-sensitive LOV2 domain that expands upon illumination. We apply the light-activated caspase (Caspase-LOV) to study neurodegeneration in larval and adult
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.