ArticleProceedings of the National Academy of Sciences of the United States of America2017
Nanoswitch-linked immunosorbent assay (NLISA) for fast, sensitive, and specific protein detection.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 89 citations in OpenAlex.
- DISCO-LAMP: a novel discontinuous LAMP assay for isothermal antigen detection.Sensors & diagnostics · 2026Article
- Nonenzymatic Detection of SARS-CoV‑2 RNA Using DNA Nanoswitches.ACS nano medicine · 2026Article
- Aptamer-based DNA nanoswitches for multiplexed protein detection.Chemical communications (Cambridge, England) · 2026Article
- A 3D Printed Mini-Gel Electrophoresis System for Rapid and Inexpensive DNA Nanoswitch Biosensing.ACS measurement science au · 2026Article
- Ligation-assisted target recycling for DNA nanoswitch biosensors.bioRxiv : the preprint server for biology · 2026Article
- Aptamer-based DNA nanoswitches for multiplexed protein detection.bioRxiv : the preprint server for biology · 2026Article
- A 3D printed mini-gel electrophoresis system for rapid and inexpensive DNA nanoswitch biosensing.bioRxiv : the preprint server for biology · 2026Article
- Modular DNA origami-based electrochemical detection of DNA and proteins.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Catalytic Hairpin Assembly-Based Self-Ratiometric Gel Electrophoresis Detection Platform for Reliable Nucleic Acid Analysis.Biosensors · 2024Article
- A mutation-sensitive, multiplexed and amplification-free detection of nucleic acids by stretching single-molecule tandem hairpin probes.Nucleic acids research · 2023Article
- Resolving Molecular Heterogeneity with Single-Molecule Centrifugation.Journal of the American Chemical Society · 2023Article
- Improved immunoassay sensitivity and specificity using single-molecule colocalization.Nature communications · 2022Article
- Single species RNA purification using DNA nanoswitches.Trends in biochemical sciences · 2022Article
- Electrophoretic mobility shift as a molecular beacon-based readout for miRNA detection.Biosensors & bioelectronics · 2021Article
- Reprogrammable Gel Electrophoresis Detection Assay Using CRISPR-Cas12a and Hybridization Chain Reaction.Analytical chemistry · 2021Article
- Direct Kinetic Fingerprinting for High-Accuracy Single-Molecule Counting of Diverse Disease Biomarkers.Accounts of chemical research · 2021Article
- DNA Nanoswitch Barcodes for Multiplexed Biomarker Profiling.Nano letters · 2021Article
- Proofreading through spatial gradients.eLife · 2020Article
- Microfluidic devices for the detection of viruses: aspects of emergency fabrication during the COVID-19 pandemic and other outbreaks.Proceedings. Mathematical, physical, and engineering sciences · 2020Review
- Direct kinetic fingerprinting and digital counting of single protein molecules.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Protein detection and quantification play critical roles in both basic research and clinical practice. Current detection platforms range from the widely used ELISA to more sophisticated, and more expensive, approaches such as digital ELISA. Despite advances, there remains a need for a method that combines the simplicity and cost-effectiveness of ELISA with the sensitivity and speed of modern approaches in a format suitable for both laboratory and rapid, point-of-care applications. Building on recent developments in DNA structural nanotechnology, we introduce the nanoswitch-linked immunosorbent assay (NLISA), a detection platform based on easily constructed DNA nanodevices that change conformation upon binding to a target protein with the results read out by gel electrophoresis. NLISA is surface-free and includes a kinetic-proofreading step for purification, enabling both enhanced sensitivity and reduced cross-reactivity. We demonstrate femtomolar-level detection of prostate-specific antigen in biological fluids, as well as reduced cross-reactivity between different serotypes of dengue and also between a single-mutation and wild-type protein. NLISA is less expensive, uses less sample volume, is more rapid, and, with no washes, includes fewer hands-on steps than ELISA, while also achieving superior sensitivity. Our approach also has the potential to enable rapid point-of-care assays, as we demonstrate by performing NLISA with an iPad/iPhone camera for imaging.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.