ArticleJournal of lipid research2017
HDL structure and function is profoundly affected when stored frozen in the absence of cryoprotectants.
Article in Journal of lipid research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed.
- Diets Low in Saturated Fat with Different Unsaturated Fatty Acid Profiles Similarly Increase Serum-Mediated Cholesterol Efflux from THP-1 Macrophages in a Population with or at Risk for Metabolic Syndrome: The Canola Oil Multicenter Intervention Trial.The Journal of nutrition · 2018Trial
- Assessment of Biochemical Analytes Stability: The Role of Centrifugation and Storage Conditions in the Preanalytical Phase.Analytical science advances · 2026Article
- HDL from 36-h fasted participants potently promotes efflux of cholesteryl ester from activated microglia.Frontiers in aging neuroscience · 2025Article
- Low concentrations of medium-sized HDL particles predict incident CVD in chronic kidney disease patients.Journal of lipid research · 2023Article
- Everolimus-Loaded Reconstituted High-Density Lipoprotein Prepared by a Novel Dual Centrifugation Approach for Anti-Atherosclerotic Therapy.International journal of nanomedicine · 2022Article
- Anti-inflammatory HDL effects are impaired in atrial fibrillation.Heart and vessels · 2022Article
- High-Density Lipoprotein Subfractions: Much Ado about Nothing or Clinically Important?Biomedicines · 2021Review
- Common and Novel Markers for Measuring Inflammation and Oxidative Stress Ex Vivo in Research and Clinical Practice-Which to Use Regarding Disease Outcomes?Antioxidants (Basel, Switzerland) · 2021Review
- Lysis reagents, cell numbers, and calculation method influence high-throughput measurement of HDL-mediated cholesterol efflux capacity.Journal of lipid research · 2021Article
- Current Understanding of the Relationship of HDL Composition, Structure and Function to Their Cardioprotective Properties in Chronic Kidney Disease.Biomolecules · 2020Review
- CKD Increases Carbonylation of HDL and Is Associated with Impaired Antiaggregant Properties.Journal of the American Society of Nephrology : JASN · 2020Article
- Reduced Reverse Cholesterol Transport Efficacy in Healthy Men with Undesirable Postprandial Triglyceride Response.Biomolecules · 2020Article
- Altered composition and functional profile of high-density lipoprotein in leprosy patients.PLoS neglected tropical diseases · 2020Article
- Neonatal HDL Counteracts Placental Vascular Inflammation via S1P-S1PR1 Axis.International journal of molecular sciences · 2020Article
- Deepening our understanding of HDL proteome.Expert review of proteomics · 2019Review
- Abnormal composition and function of high-density lipoproteins in atopic dermatitis patients.Allergy · 2019Article
- Cholesterol efflux responds to viral load and CD4 counts in HIV+ patients and is dampened in HIV exposed.Journal of lipid research · 2018Article
- HDL functionality and cardiovascular outcome among nondialysis chronic kidney disease patients.Journal of lipid research · 2018Article
- Lipid composition dictates serum stability of reconstituted high-density lipoproteins: implications for in vivo applications.Nanoscale · 2018Article
- Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Analysis of structural and functional parameters of HDL has gained significant momentum in recent years because they are stronger predictors of cardiovascular risk than HDL-cholesterol levels. Surprisingly, in most HDL studies, very low attention is paid to HDL storage, which might critically affect functional properties. In the present study, we systematically examined the impact of storage and freezing on the structural/functional properties of freshly isolated HDL. Initial damage to HDL starts between week 1 and week 4 of storage. We observed that prolonged freezing at -20°C or -70°C led to a shedding of apoA-I from HDL and to the formation of large protein-poor particles, indicating that HDL is irreversibly disrupted. These structural alterations profoundly affected key metrics of HDL function, including HDL-cholesterol efflux capacity and HDL paraoxonase activity. Flash-freezing of isolated HDL prior to storage at -70°C did not preserve HDL structure. However, addition of the cryoprotectants, sucrose or glycerol, completely preserved structure and function of HDL when stored for at least 2 years. Our data clearly indicate that HDL is a complex particle requiring special attention when stored. Addition of cryoprotectants to isolated HDL samples before storage will make biochemical and clinical HDL research studies more reproducible and comparable.
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