ArticleACS combinatorial science2017
Library Design-Facilitated High-Throughput Sequencing of Synthetic Peptide Libraries.
Article in ACS combinatorial science, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed.
- Affinity selection mass spectrometry screening discovers new COP1 peptide binders.RSC chemical biology · 2026Article
- Massive barcode-free chemical screenings enable the discovery of bioactive macrocycles with passive membrane permeability.Nature communications · 2026Article
- Barcode-free hit discovery from massive libraries enabled by automated small molecule structure annotation.Nature communications · 2025Article
- Engineered GLP-1R-targeting nanoplatforms: multimodal therapeutics in human diseases.Journal of nanobiotechnology · 2025Review
- Affinity selection-mass spectrometry with linearizable macrocyclic peptide libraries.Science advances · 2025Article
- Advances in Ultrahigh Throughput Hit Discovery with Tandem Mass Spectrometry Encoded Libraries.Journal of the American Chemical Society · 2023Review
- In-Cell Penetration Selection-Mass Spectrometry Produces Noncanonical Peptides for Antisense Delivery.ACS chemical biology · 2023Article
- Abiotic peptides as carriers of information for the encoding of small-molecule library synthesis.Science (New York, N.Y.) · 2023Article
- Binary combinatorial scanning reveals potent poly-alanine-substituted inhibitors of protein-protein interactions.Communications chemistry · 2022Article
- Rapid de novo discovery of peptidomimetic affinity reagents for human angiotensin converting enzyme 2.Communications chemistry · 2022Article
- Tuning of a Membrane-Perforating Antimicrobial Peptide to Selectively Target Membranes of Different Lipid Composition.The Journal of membrane biology · 2021Article
- Article
- Discovery of Nucleic Acid Binding Molecules from Combinatorial Biohybrid Nucleobase Peptide Libraries.Journal of the American Chemical Society · 2020Article
- Secondary Amino Alcohols: Traceless Cleavable Linkers for Use in Affinity Capture and Release.Angewandte Chemie (International ed. in English) · 2020Article
- Ultra-large chemical libraries for the discovery of high-affinity peptide binders.Nature communications · 2020Article
- Evolving a Peptide: Library Platforms and Diversification Strategies.International journal of molecular sciences · 2019Review
- Quantitative mapping of protein-peptide affinity landscapes using spectrally encoded beads.eLife · 2019Article
- In-solution enrichment identifies peptide inhibitors of protein-protein interactions.Nature chemical biology · 2019Article
- Xenoprotein engineering via synthetic libraries.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Site-Selective Cysteine-Cyclooctyne Conjugation.Angewandte Chemie (International ed. in English) · 2018Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
A methodology to achieve high-throughput de novo sequencing of synthetic peptide mixtures is reported. The approach leverages shotgun nanoliquid chromatography coupled with tandem mass spectrometry-based de novo sequencing of library mixtures (up to 2000 peptides) as well as automated data analysis protocols to filter away incorrect assignments, noise, and synthetic side-products. For increasing the confidence in the sequencing results, mass spectrometry-friendly library designs were developed that enabled unambiguous decoding of up to 600 peptide sequences per hour while maintaining greater than 85% sequence identification rates in most cases. The reliability of the reported decoding strategy was additionally confirmed by matching fragmentation spectra for select authentic peptides identified from library sequencing samples. The methods reported here are directly applicable to screening techniques that yield mixtures of active compounds, including particle sorting of one-bead one-compound libraries and affinity enrichment of synthetic library mixtures performed in solution.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.