Evidence map›Paper›PMID 28889290›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2017

Live Flow Cytometry Analysis of c-di-GMP Levels in Single Cell Populations.

Jongchan Yeo, Xin C Wang, Ming C Hammond

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.4field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Jongchan YeoDepartment of Chemistry, University of California, Berkeley, 94720, USA.
Xin C WangDepartment of Molecular and Cell Biology, University of California, Berkeley, 94720, USA.
Ming C HammondDepartment of Chemistry, University of California, Berkeley, 94720, USA. mingch@berkeley.edu.
University of California, Berkeley · US

Funding

A Chemical Biology Approach to Tagging RNAs in Live CellsDP2OD008677 · OD · UNIVERSITY OF CALIFORNIA BERKELEY · PI HAMMOND, MING CHEN · 2011 to 2011
$2.3M
NIH HHS DP2 OD008677
6 · The paper itself

Abstract

Second-generation RNA-based fluorescent biosensors have been developed that enable flow cytometry experiments to monitor the population dynamics of c-di-GMP signaling in live bacteria. These experiments are high-throughput, provide information at the single-cell level, and can be performed on cells grown in complex media and/or under anaerobic conditions. Here, we describe flow cytometry methods for three applications: (1) high-throughput screening for diguanylate cyclase activity, (2) analyzing c-di-GMP levels under anaerobic conditions, and (3) monitoring cell population dynamics of c-di-GMP levels upon environmental changes. These methods showcase RNA-based fluorescent biosensors as versatile tools for studying c-di-GMP signaling in bacteria.

Indexed as

Flow CytometrySingle-Cell AnalysisAnaerobiosisBiosensing TechniquesCyclic GMPEnzyme ActivationEscherichia coliEscherichia coli ProteinsPhosphorus-Oxygen Lyasesbis(3',5')-cyclic diguanylic acidCyclic GMPdiguanylate cyclaseEscherichia coli ProteinsPhosphorus-Oxygen LyasesAnaerobic growthC-di-GMP signalingCyclic di-GMPCyclic dinucleotideFlow cytometryRNA-based fluorescent biosenso rSpinach aptame r

Identifiers

PMID28889290
PMCPMC5942886
OpenAlexW2754396002

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.