Evidence map›Paper›PMID 28889081›Full record

ArticleBiomaterials2017

Distinct solubility and cytotoxicity regimes of paclitaxel-loaded cationic liposomes at low and high drug content revealed by kinetic phase behavior and cancer cell viability studies.

Victoria M Steffes, Meena M Murali, Yoonsang Park, Bretton J Fletcher, Kai K Ewert, Cyrus R Safinya

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
2.5field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 52 citations in OpenAlex.

  1. Article
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  5. Article
  6. Pharmacokinetic behaviors of soft nanoparticulate formulations of chemotherapeutics.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2023
    Review
  7. Article
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  13. Article
  14. Three-Dimensional Microphase Separation and Synergistic Permeability in Stacked Lipid-Polymer Hybrid Membranes.Chemistry of materials : a publication of the American Chemical Society · 2017
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Victoria M SteffesChemistry and Biochemistry Department, University of California, Santa Barbara, CA 93106, USA; Materials Department, University of California, Santa Barbara, CA 93106, USA.
Meena M MuraliMaterials Department, University of California, Santa Barbara, CA 93106, USA.
Yoonsang ParkMaterials Department, University of California, Santa Barbara, CA 93106, USA.
Bretton J FletcherMaterials Department, University of California, Santa Barbara, CA 93106, USA.
Kai K EwertMaterials Department, University of California, Santa Barbara, CA 93106, USA.
Cyrus R SafinyaMaterials Department, University of California, Santa Barbara, CA 93106, USA; Physics Department, University of California, Santa Barbara, CA 93106, USA; Molecular, Cellular & Developmental Biology Department, University of California, Santa Barbara, CA 93106, USA. Electronic address: Safinya@mrl.ucsb.edu.
University of California, Santa Barbara · US

Funding

Structures of Lipid-DNA Nonviral Gene Delivery Systems 4R01GM059288 · NIGMS · UNIVERSITY OF CALIFORNIA SANTA BARBARA · PI SAFINYA, CYRUS R · 1999 to 2016
$4.2M
NIGMS NIH HHS R01 GM059288
6 · The paper itself

Abstract

Lipid-based particles are used worldwide in clinical trials as carriers of hydrophobic paclitaxel (PTXL) for cancer chemotherapy, albeit with little improvement over the standard-of-care. Improving efficacy requires an understanding of intramembrane interactions between PTXL and lipids to enhance PTXL solubilization and suppress PTXL phase separation into crystals. We studied the solubility of PTXL in cationic liposomes (CLs) composed of positively charged 2,3-dioleyloxypropyltrimethylammonium chloride (DOTAP) and neutral 1,2-dioleoyl-sn-glycero-3-phosphatidylcholine (DOPC) as a function of PTXL membrane content and its relation to efficacy. Time-dependent kinetic phase diagrams were generated from observations of PTXL crystal formation by differential-interference-contrast microscopy. Furthermore, a new synchrotron small-angle x-ray scattering in situ methodology applied to DOTAP/DOPC/PTXL membranes condensed with DNA enabled us to detect the incorporation and time-dependent depletion of PTXL from membranes by measurements of variations in the membrane interlayer and DNA interaxial spacings. Our results revealed three regimes with distinct time scales for PTXL membrane solubility: hours for >3 mol% PTXL (low), days for ≈ 3 mol% PTXL (moderate), and ≥20 days for < 3 mol% PTXL (long-term). Cell viability experiments on human cancer cell lines using CL

Indexed as

CationsCell DeathCell Line, TumorCell SurvivalCrystallizationDNAHumansKineticsLiposomesNeoplasmsPaclitaxelScattering, Small AngleSolubilitySynchrotronsX-Ray DiffractionCationsDNALiposomesPaclitaxelCancer chemotherapyDrug deliveryHydrophobic drugLiposomeNanoparticlePaclitaxel

Identifiers

PMID28889081
PMCPMC5610109
OpenAlexW2949802189

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.