Evidence map›Paper›PMID 28887493›Full record

ArticleScientific reports2017

Molecular characterization of cell-free eccDNAs in human plasma.

Jing Zhu, Fan Zhang, Meijun Du, Peng Zhang, Songbin Fu, Liang Wang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.

0numbers the graph read from it
0cells of the map it votes in
76citing papers in PubMed
2.1field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

76 citing papers in PubMed, 130 citations in OpenAlex.

  1. Article
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  3. Targeting extrachromosomal DNA in human cancers.Nature reviews. Drug discovery · 2026
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16 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 2 countries.

Jing ZhuLaboratory of Medical Genetics, Harbin Medical University, Harbin, Heilongjiang, 150081, China.
Fan ZhangSchool of Computer Science and Technology, Harbin Institute of Technology, Harbin, Heilongjiang, 150001, China.
Meijun DuDepartment of Pathology and MCW Cancer Center, Medical College of Wisconsin, Milwaukee, WI, 53226, USA.
Peng ZhangDepartment of Pathology and MCW Cancer Center, Medical College of Wisconsin, Milwaukee, WI, 53226, USA.
Songbin FuLaboratory of Medical Genetics, Harbin Medical University, Harbin, Heilongjiang, 150081, China.
Liang WangDepartment of Pathology and MCW Cancer Center, Medical College of Wisconsin, Milwaukee, WI, 53226, USA. liwang@mcw.edu.ORCID 0000-0002-9364-8572
Medical College of Wisconsin · USHarbin Medical University · CNHarbin Institute of Technology · CN

Funding

Cell free nucleic acid-based biomarkers in advanced prostate cancerR01CA212097 · NCI · MEDICAL COLLEGE OF WISCONSIN · PI KOHLI, MANISH, WANG, LIANG · 2017 to 2022
$3.1M
Genetic determinants of gene expression phenotypes in aggressive prostate cancerR01CA157881 · NCI · MEDICAL COLLEGE OF WISCONSIN · PI WANG, LIANG · 2011 to 2016
$2.9M
NCI NIH HHS R01 CA157881NCI NIH HHS R01 CA212097
6 · The paper itself

Abstract

Extrachromosomal circular DNAs (eccDNAs) have been reported in most eukaryotes. However, little is known about the cell-free eccDNA profiles in circulating system such as blood. To characterize plasma cell-free eccDNAs, we performed sequencing analysis in 26 libraries from three blood donors and negative controls. We identified thousands of unique plasma eccDNAs in the three subjects. We observed proportional eccDNA increase with initial DNA input. The detected eccDNAs were also associated with circular DNA enrichment efficiency. Increasing the sequencing depth in an additional sample identified many more eccDNAs with highly heterogenous molecular structure. Size distribution of eccDNAs varied significantly from 31 bp to 19,989 bp. We found significantly higher GC content in smaller eccDNAs (<500 bp) than the larger ones (>500 bp) (p < 0.01). We also found an enrichment of eccDNAs at exons and 3'UTR (enrichment folds from 1.36 to 3.1) as well as the DNase hypersensitive sites (1.58-2.42 fold), H3K4Me1 (1.23-1.42 fold) and H3K27Ac (1.33-1.62 fold) marks. Junction sequence analysis suggested fundamental role of nonhomologous end joining mechanism during eccDNA formation. Further characterization of the extracellular eccDNAs in peripheral blood will facilitate understanding of their molecular mechanisms and potential clinical utilities.

Indexed as

Base CompositionBiomarkersCell-Free Nucleic AcidsDNA, CircularHumansNucleotide MotifsBiomarkersCell-Free Nucleic AcidsDNA, Circular

Identifiers

PMID28887493
PMCPMC5591271
OpenAlexW2753430590

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.