Evidence map›Paper›PMID 28886289›Full record

ArticleBioengineered2018

Optimization of saccharification potential of recombinant xylanase from Bacillus licheniformis.

Muhammad N Aftab, Asma Zafar, Irfana Iqbal, Afshan Kaleem, Khalid M Zia, Ali R Awan

Open access · goldAbstract read
In one paragraph

Article in Bioengineered, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.4field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Article
  3. Expression inInternational journal of molecular sciences · 2024
    Article
  4. Article
  5. Inhibitory activity ofBioengineered · 2022
    Article
  6. Review
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 1 country.

Muhammad N Aftaba Institute of Industrial Biotechnology , Government College University , Katchery Road, Lahore , Pakistan.
Asma Zafara Institute of Industrial Biotechnology , Government College University , Katchery Road, Lahore , Pakistan.
Irfana Iqbalb Department of Zoology , Lahore College for Women University , Lahore , Pakistan.
Afshan Kaleemc Department of Biotechnology , Lahore College for Women University , Lahore , Pakistan.
Khalid M Ziad Institute of Chemistry , Government College University , Faisalabad , Pakistan.
Ali R Awane Institute of Biochemistry & Biotechnology , University of Veterinary and Animal Sciences , Lahore , Pakistan.
Government College University, Lahore · PKLahore College for Women University · PKGovernment College University, Faisalabad · PKUniversity of Veterinary and Animal Sciences · PK

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Saccharification potential of xylanase enzyme cloned from Bacillus licheniformis into E. coli BL21 (DE3) was evaluated against plant biomass for the production of bioethanol. The expression of cloned gene was studied and conditions were optimized for its large scale production. The parameters effecting enzyme production were examined in a fermenter. Recombinant xylanase has the ability to breakdown birchwood xylan to release xylose as well as the potential to treat plant biomass, such as wheat straw, rice straw, and sugarcane bagass. The saccharification ability of this enzyme was optimized by studying various parameters. The maximum saccharification percentage (84%) was achieved when 20 units of recombinant xylanase were used with 8% sugarcane bagass at 50°C and 120 rpm after 6 hours of incubation. The results indicated that the bioconversion of natural biomass by recombinant xylanase into simple sugars can be used for biofuel (bioethanol) production. This process can replace the use of fossil fuels, and the use of bioethanol can significantly reduce the emission of toxic gases. Future directions regarding pre-treatment of cellulosic and hemicellulosic biomass and other processes that can reduce the cost and enhance the yield of biofuels are briefly discussed.

Indexed as

Bacillus licheniformisBacterial ProteinsBiofuelsBioreactorsCelluloseCloning, MolecularEndo-1,4-beta XylanasesEscherichia coliEthanolFermentationGene ExpressionGenetic VectorsHumansRecombinant ProteinsSaccharumXylansBacterial ProteinsbagasseBiofuelsCelluloseEndo-1,4-beta XylanasesEthanolRecombinant ProteinsXylansbiomassexpressionsaccharificationXylanase

Identifiers

PMID28886289
PMCPMC5972937
OpenAlexW2753604809

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.