Evidence map›Paper›PMID 28886265›Full record

ArticleCancer biology & therapy2017

Microvesicles releasing by oral cancer cells enhance endothelial cell angiogenesis via Shh/RhoA signaling pathway.

Xiao Huaitong, Feng Yuanyong, Tao Yueqin, Zhao Peng, Shang Wei, Song Kai

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancer biology & therapy, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed, 1 pooled it
2.4field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 1 synthesis or guideline pooled it, 45 citations in OpenAlex.

  1. Pooled it
  2. Review
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  5. Review
  6. MYCN in human development and diseases.Frontiers in oncology · 2024
    Review
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  19. Article
  20. The Microenvironment of Tongue Cancer.Advances in experimental medicine and biology · 2020
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Xiao Huaitonga School of Stomatology , Qingdao University , Shandong Province , China.
Feng Yuanyonga School of Stomatology , Qingdao University , Shandong Province , China.
Tao Yueqina School of Stomatology , Qingdao University , Shandong Province , China.
Zhao Penga School of Stomatology , Qingdao University , Shandong Province , China.
Shang Weia School of Stomatology , Qingdao University , Shandong Province , China.
Song Kaia School of Stomatology , Qingdao University , Shandong Province , China.
Qingdao University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The present study aimed to investigate the significance of hedgehog signaling pathway in association with clinicopathology parameters and its effect on angiogenesis in oral squamous cell carcinoma (OSCC). The expression of Sonic Hh (Shh) and Gli1 were done on primary tumors and metastatic lymph nodes in OSCC samples from 80 patients by immunohistochemical analysis. The western blot was used to examine the expression of Shh in OSCC cell lines and OSCC-derived microvesicles (MVs). The role of Shh carried by MVs to induce endothelial cell angiogenesis was further investigated by matrigel assay. Our results indicated that the expression of Shh was positive associated with microvesseldentisty(MVD), TNM stage, tumor recurrence and lymph node metastasis. Moreover, Shh and Gli1 expression were higher in paired metastatic lymph nodes compared with expression of their primary tumors. The expression of Shh was abundant in Cal27, and present in SCC4, SCC9, and the amount of Shh protein in Cal27 targeting MVs was increased significantly than Cal27 cell group, up to ∼ fifth-fold. The Cal27 derived MVs increased significantly angiogenesis of HUVECs in vitro, and this effect was blocked with exoenzyme C3 transferase (C3) and shRNA targeting RhoA by suppressing RhoA expression and activation. The data suggested that OSCC derived Shh carried by MVs may facilitate the tumor growth and modulate the preparation of a vascular network in primary tumor and/or premetastatic niche.

Indexed as

ADP Ribose TransferasesBiomarkers, TumorBotulinum ToxinsCarcinoma, Squamous CellCell-Derived MicroparticlesCell Line, TumorEndothelial CellsFemaleGene Knockdown TechniquesHedgehog ProteinsHumansHuman Umbilical Vein Endothelial CellsLymphatic MetastasisLymph NodesMaleMicrovesselsADP Ribose TransferasesBiomarkers, TumorBotulinum Toxinsexoenzyme C3, Clostridium botulinumGLI1 protein, humanHedgehog ProteinsrhoA GTP-Binding ProteinRHOA protein, humanRNA, Small InterferingSHH protein, humanZinc Finger Protein GLI1angiogenesisendothelial cellsGli1microvesiclesOral cancerRhoAShh

Identifiers

PMID28886265
PMCPMC5678693
OpenAlexW2751586138

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.