ArticleALTEX2018
Ex vivo model unravelling cell distribution effect in hydrogels for cartilage repair.
Article in ALTEX, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 31 citations in OpenAlex.
- Animal Models of Osteoarthritis: Updated Models and Outcome Measures 2016-2023.Regenerative engineering and translational medicine · 2024Article
- Isolation of Chondrons from Hyaline Cartilage.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Gelatin methacryloyl as environment for chondrocytes and cell delivery to superficial cartilage defects.Journal of tissue engineering and regenerative medicine · 2022Article
- 3D Printed Multiphasic Scaffolds for Osteochondral Repair: Challenges and Opportunities.International journal of molecular sciences · 2021Review
- Article
- An ex vivo human osteochondral culture model.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2021Article
- Development of anCartilage · 2021Article
- Applications of Biocompatible Scaffold Materials in Stem Cell-Based Cartilage Tissue Engineering.Frontiers in bioengineering and biotechnology · 2021Review
- One-Step Photoactivation of a Dual-Functionalized Bioink as Cell Carrier and Cartilage-Binding Glue for Chondral Regeneration.Advanced healthcare materials · 2020Article
- Article
- The evaluation of oxidative stress in osteoarthritis.Medicine and pharmacy reports · 2020Review
- 3D bioprinting via an in situ crosslinking technique towards engineering cartilage tissue.Scientific reports · 2019Article
- Protocols for Culturing and Imaging a HumanMaterials (Basel, Switzerland) · 2019Article
- Models of osteoarthritis: the good, the bad and the promising.Osteoarthritis and cartilage · 2019Review
- Synthesis and Properties of Gelatin Methacryloyl (GelMA) Hydrogels and Their Recent Applications in Load-Bearing Tissue.Polymers · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The implantation of chondrocyte-laden hydrogels is a promising cartilage repair strategy. Chondrocytes can be spatially positioned in hydrogels and thus in defects, while current clinical cell therapies introduce chondrocytes in the defect depth. The main aim of this study was to evaluate the effect of spatial chondrocyte distribution on the reparative process. To reduce animal experiments, an ex vivo osteochondral plug model was used and evaluated. The role of the delivered and endogenous cells in the repair process was investigated. Full thickness cartilage defects were created in equine osteochondral plugs. Defects were filled with (A) chondrocytes at the bottom of the defect, covered with a cell-free hydrogel, (B) chondrocytes homogeneously encapsulated in a hydrogel, and (C, D) combinations of A and B with different cell densities. Plugs were cultured for up to 57 days, after which the cartilage and repair tissues were characterized and compared to baseline samples. Additionally, at day 21, the origin of cells in the repair tissue was evaluated. Best outcomes were obtained with conditions C and D, which resulted in well-integrated cartilage-like tissue that completely filled the defect, regardless of the initial cell density. A critical role of the spatial chondrocyte distribution in the repair process was observed. Moreover, the osteochondral plugs stimulated cartilage formation in the hydrogels when cultured in the defects. The resulting repair tissue originated from the delivered cells. These findings confirm the potential of the osteochondral plug model for the optimization of the composition of cartilage implants and for studying repair mechanisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.