Evidence map›Paper›PMID 28883894›Full record

Trial reportClinical epigenetics2017

Epigenetic regulation of inflammation in localized aggressive periodontitis.

L M Shaddox, A F Mullersman, H Huang, S M Wallet, T Langaee, I Aukhil

Registry-linked trialOpen access · goldAbstract readClinical Trial
PubMed Full text at PMC Publisher
In one paragraph

Trial report in Clinical epigenetics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT01330719 (Mechanisms and Treatment Response of Aggressive Periodontitis in Children), which is not on this map. Cited by 24 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed, 2 pooled it
4.2field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT01330719 nacompletednot on this map

Mechanisms and Treatment Response of Aggressive Periodontitis in Children: Aberrant Immunological Phenotypes/Functions in the Progression of AgP

TypeinterventionalSponsorUniversity of FloridaRan2006 to 2022Enrolled514ConditionsAggressive PeriodontitisArmsDiseased periodontal treatment, Conventional periodontal treatment
3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 2 syntheses or guidelines pooled it, 46 citations in OpenAlex.

  1. Pooled it
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  14. The Molecular Comorbidity Network of Periodontal Disease.International journal of molecular sciences · 2024
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

L M ShaddoxDepartment of Periodontology, University of Florida College of Dentistry, P.O. Box 100434, Gainesville, FL 32610-0434 USA.
A F MullersmanDepartment of Periodontology, University of Florida College of Dentistry, P.O. Box 100434, Gainesville, FL 32610-0434 USA.
H HuangDepartment of Periodontology, University of Florida College of Dentistry, P.O. Box 100434, Gainesville, FL 32610-0434 USA.
S M WalletDepartment of Oral Biology, University of Florida College of Dentistry, Gainesville, FL USA.
T LangaeeCenter for Pharmacogenomics, University of Florida, Gainesville, FL USA.
I AukhilDepartment of Periodontology, University of Florida College of Dentistry, P.O. Box 100434, Gainesville, FL 32610-0434 USA.
Florida College · USUniversity of Florida · US

Funding

Mechanisms and Treatment Response of Aggressive Periodontitis in ChildrenR01DE019456 · NIDCR · UNIVERSITY OF FLORIDA · PI AUKHIL, IKRAMUDDIN, SHADDOX, LUCIANA MACCHION · 2009 to 2018
$5.6M
Tissue-Targeted Enzyme for Localized Tryptophan Catabolism to Direct Subcutaneous and Oral Mucosal Inflammatory ResponsesR01DE027301 · NIDCR · UNIVERSITY OF FLORIDA · PI KESELOWSKY, BENJAMIN GEORGE · 2017 to 2020
$2.0M
Comprehensive Training Program in Oral BiologyR90DE022530 · NIDCR · UNIVERSITY OF FLORIDA · PI LEMOS, JOSE A · 2011 to 2025
$995k
NIDCR NIH HHS R01 DE019456NIDCR NIH HHS R01 DE027301NIDCR NIH HHS R90 DE022530
6 · The paper itself

Abstract

backgroundWe have previously demonstrated a Toll-like receptor (TLR)-mediated hyper-responsive phenotype in our cohort of localized aggressive periodontitis (LAP) individuals. However, mechanisms related to this phenotype are still not clear in the literature. The objective of this cross-sectional study is to examine the role of epigenetic regulation, specifically DNA methylation status of genes in the TLR pathway in this cohort. Peripheral blood was collected from 20 LAP patients and 20 healthy unrelated controls. Whole blood was stimulated with 1 μl (100 ng/μl) of purified

resultsSignificant differences in the methylation status were observed at specific CpG positions in LAP patients compared to healthy controls and interestingly also between severe and moderate LAP. Specifically, subjects with moderate LAP presented hypermethylation of both the upregulating (MAP3K7, MYD88, IL6R, and RIPK2) and downregulating (FADD, IRAK, and PPARA) genes, while severe LAP presented hypomethylation of these genes. Further analysis on CpG sites with significant differences in methylation status correlates with an increased pro-inflammatory cytokine profile for LAP patients.

conclusionsOur findings suggest that epigenetic modifications of genes in the TLR pathway may orchestrate the thresholds for balancing induction and prevention of tissue destruction during the course of disease, and thus differ significantly at different stages of the disease, where moderate LAP shows hypermethylation and severe LAP shows hypomethylation of several genes.

trial registrationhttps://clinicaltrials.gov, NCT01330719.

Indexed as

DNA MethylationGene Regulatory NetworksAdolescentAggressive PeriodontitisCase-Control StudiesChildCross-Sectional StudiesCytokinesEpigenesis, GeneticFemaleHumansMaleSequence Analysis, DNASignal TransductionToll-Like ReceptorsYoung AdultCytokinesToll-Like ReceptorsAggressive periodontitisEpigeneticsInflammationLeukotoxinsToll-like receptors

Identifiers

PMID28883894
PMCPMC5581417
OpenAlexW2753014927

What OpenQuestion holds

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LicenceCC BY
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Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.