ArticleNature communications2017
Engineered proteins with sensing and activating modules for automated reprogramming of cellular functions.
Article in Nature communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 33 citations in OpenAlex.
- Perspectives on Synthetic Protein Circuits in Mammalian Cells.Current opinion in biomedical engineering · 2024Article
- Nanosystem Delivers Senescence Activators and Immunomodulators to Combat Liver Cancer.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Cooperative phagocytosis of solid tumours by macrophages triggers durable anti-tumour responses.Nature biomedical engineering · 2023Article
- Matrix stiffness, endothelial dysfunction and atherosclerosis.Molecular biology reports · 2023Review
- Engineering SH2 Domains with Tailored Specificities and Affinities.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Mechanosensor Piezo1 mediates bimodal patterns of intracellular calcium and FAK signaling.The EMBO journal · 2022Article
- Therapeutic cell engineering: designing programmable synthetic genetic circuits in mammalian cells.Protein & cell · 2022Review
- Monocytes engineered with iSNAP inhibit human B-lymphoma progression.Bioengineering & translational medicine · 2022Article
- Engineering CAR T cells for enhanced efficacy and safety.APL bioengineering · 2022Review
- Resource for FRET-Based Biosensor Optimization.Frontiers in cell and developmental biology · 2022Review
- Phosphoregulated orthogonal signal transduction in mammalian cells.Nature communications · 2020Article
- Engineering light-controllable CAR T cells for cancer immunotherapy.Science advances · 2020Article
- Tumor-Associated Macrophages: Recent Insights and Therapies.Frontiers in oncology · 2020Review
- Article
- Protein circuits reprogram cells.Nature chemical biology · 2019Article
- Group AMicrobiology spectrum · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
15 authors at 2 institutions in 3 countries.
Funding
Abstract
Protein-based biosensors or activators have been engineered to visualize molecular signals or manipulate cellular functions. Here we integrate these two functionalities into one protein molecule, an integrated sensing and activating protein (iSNAP). A prototype that can detect tyrosine phosphorylation and immediately activate auto-inhibited Shp2 phosphatase, Shp2-iSNAP, is designed through modular assembly. When Shp2-iSNAP is fused to the SIRPα receptor which typically transduces anti-phagocytic signals from the 'don't eat me' CD47 ligand through negative Shp1 signaling, the engineered macrophages not only allow visualization of SIRPα phosphorylation upon CD47 engagement but also rewire the CD47-SIRPα axis into the positive Shp2 signaling, which enhances phagocytosis of opsonized tumor cells. A second SIRPα Syk-iSNAP with redesigned sensor and activator modules can likewise rewire the CD47-SIRPα axis to the pro-phagocytic Syk kinase activation. Thus, our approach can be extended to execute a broad range of sensing and automated reprogramming actions for directed therapeutics.Protein-based biosensors have been engineered to interrogate cellular signaling and manipulate function. Here the authors demonstrate iSNAP, a tool to detect tyrosine phosphorylation and activate desired protein enzymes allowing the control of phagocytosis in macrophages.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.