ArticleScientific reports2017
Early X chromosome inactivation during human preimplantation development revealed by single-cell RNA-sequencing.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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59 citing papers in PubMed, 111 citations in OpenAlex.
- XIST drives X-chromosome inactivation and safeguards female extraembryonic cells in humans.Nature communications · 2026Article
- Neural peer pressure: intercellular dynamics and emergent phenotypes in the mosaic Rett syndrome brain.Cell communication and signaling : CCS · 2026Review
- Reprogramming the inactive X chromosome: dynamics and insights from the germline.Biochemical Society transactions · 2026Review
- Sex-influenced DNA methylation differs by placental cell type.Biology of sex differences · 2026Article
- The role of m6A RNA methylation in the maintenance of X chromosome inactivation and X-to-autosome dosage compensation in early embryonic lineages.Stem cell reports · 2026Article
- Escape of Kdm6a from X Chromosome Is Detrimental to Ischemic Brains via IRF5 Signaling.Translational stroke research · 2025Article
- X-chromosome upregulation operates on a gene-by-gene basis at RNA and protein levels.Nature communications · 2025Article
- Single Cell RNA Sequencing and Its Impact on Understanding Human Embryo Development.International journal of molecular sciences · 2025Review
- X chromosome inactivation in mammals: general principles and species-specific considerations.EMBO reports · 2025Review
- Breaking rules: the complex relationship between DNA methylation and X-chromosome inactivation in the human placenta.Biology of sex differences · 2025Article
- Lineage-specific dynamics of loss of X upregulation during inactive-X reactivation.Stem cell reports · 2024Article
- Population variability in X-chromosome inactivation across 10 mammalian species.Nature communications · 2024Article
- Escape of Kdm6a from X chromosome is detrimental to ischemic brains via IRF5 signaling.Research square · 2024Article
- Compensation of gene dosage on the mammalian X.Development (Cambridge, England) · 2024Review
- Silencing XIST on the future active X: Searching human and bovine preimplantation embryos for the repressor.European journal of human genetics : EJHG · 2024Article
- Modeling X-chromosome inactivation and reactivation during human development.Current opinion in genetics & development · 2023Review
- Female naïve human pluripotent stem cells carry X chromosomes with Xa-like and Xi-like folding conformations.Science advances · 2023Article
- The compleX balancing act of controlling X-chromosome dosage and how it impacts mammalian germline development.The Biochemical journal · 2023Article
- Generation of blastoids from human parthenogenetic stem cells.Life medicine · 2023Article
- X-chromosome inactivation patterns depend on age and tissue but not conception method in humans.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2023Article
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4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In female mammals, one X chromosome is transcriptionally inactivated (XCI), leading to dosage compensation between sexes, fundamental for embryo viability. A previous study using single-cell RNA-sequencing (scRNA-seq) data proposed that female human preimplantation embryos achieve dosage compensation by downregulating both Xs, a phenomenon named dampening of X expression. Using a novel pipeline on those data, we identified a decrease in the proportion of biallelically expressed X-linked genes during development, consistent with XCI. Moreover, we show that while the expression sum of biallelically expressed X-linked genes decreases with embryonic development, their median expression remains constant, rejecting the hypothesis of X dampening. In addition, analyses of a different dataset of scRNA-seq suggest the appearance of X-linked monoallelic expression by the late blastocyst stage in females, another hallmark of initiation of XCI. Finally, we addressed the issue of dosage compensation between the single active X and autosomes in males and females for the first time during human preimplantation development, showing emergence of X to autosome dosage compensation by the upregulation of the active X chromosome in both male and female embryonic stem cells. Our results show compelling evidence of an early process of X chromosome inactivation during human preimplantation development.
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