ArticleNature communications2017
Multiscale cytometry and regulation of 3D cell cultures on a chip.
Article in Nature communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
64 citing papers in PubMed.
- Developing Micro/Nanostructured Fluidic Mixing Technology for Biomedical Applications.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- A Microwell Array Embedded Microfluidic Gradient Platform for Drug Screening on Tumor Spheroids.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Engineering Mesenchymal Stem Cell Spheroids and Brain Organoids: Advanced 3D Culture Platforms for Neurodegenerative Disease Cell Therapy.Stem cell reviews and reports · 2026Review
- High-Throughput Generation of Tumor Spheroids via Droplet Microfluidics for siRNA-Loaded Nanomedicine Assessment.Advanced healthcare materials · 2026Article
- Spherical Skin Model: Stratified Co-Culture of Fibroblasts and Keratinocytes on Spherical Beads Toward Compound Screening.Advanced healthcare materials · 2026Article
- Cell Viability, Drug Screening, and Mechanism Study Under Mild Phototherapy Integrated Chemotherapy (PIC).Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Advanced Microfluidics for Single Cell-Based Cancer Research.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Culture of pluripotent stem cells in microscale droplets modulates differentiation and tissue patterning towards organoids on chip.Stem cell research & therapy · 2025Article
- Droplet Generation and Manipulation in Microfluidics: A Comprehensive Overview of Passive and Active Strategies.Biosensors · 2025Review
- Real-time monitoring and quantitative analysis of 3D tumor spheroids using portable cellular imaging system.Biomedical engineering letters · 2025Article
- Pheno-Morphological Screening and Acoustic Sorting of 3D Multicellular Aggregates Using Drop Millifluidics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Advancing cancer research through 3D cell culture models.EXCLI journal · 2025Review
- Cancer-on-a-chip model shows that the adenomatous polyposis coli mutation impairs T cell engagement and killing of cancer spheroids.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Knockout cancer by nano-delivered immunotherapy using perfusion-aided scaffold-based tumor-on-a-chip.Nanotheranostics · 2024Review
- Spheroid Formation and Recovery Using Superhydrophobic Coating for Regenerative Purposes.Pharmaceutics · 2023Article
- Combinatorial drug screening on 3D Ewing sarcoma spheroids using droplet-based microfluidics.iScience · 2023Article
- Review
- Functional precision oncology using patient-derived assays: bridging genotype and phenotype.Nature reviews. Clinical oncology · 2023Review
- Microfluidic Chips: Emerging Technologies for Adoptive Cell Immunotherapy.Micromachines · 2023Review
- Spheroid Engineering in Microfluidic Devices.ACS omega · 2023Review
4 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Three-dimensional cell culture is emerging as a more relevant alternative to the traditional two-dimensional format. Yet the ability to perform cytometry at the single cell level on intact three-dimensional spheroids or together with temporal regulation of the cell microenvironment remains limited. Here we describe a microfluidic platform to perform high-density three-dimensional culture, controlled stimulation, and observation in a single chip. The method extends the capabilities of droplet microfluidics for performing long-term culture of adherent cells. Using arrays of 500 spheroids per chip, in situ immunocytochemistry and image analysis provide multiscale cytometry that we demonstrate at the population scale, on 10
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.