ArticleScientific reports2017
Assessing SNP genotyping of noninvasively collected wildlife samples using microfluidic arrays.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed.
- High-Density SNP Genotyping Reveals High Population Connectivity and Limited Spatial Genetic Structure inEcology and evolution · 2026Article
- Genomic Data Describe Population Structure but Struggle to Estimate Directed Connectivity Networks.Molecular ecology resources · 2026Article
- Individual Identification of Prey in Carnivore Scats.Molecular ecology resources · 2026Article
- Estimating the Abundance of a Cryptic, Endangered Marsupial, Mala (Ecology and evolution · 2026Article
- Assessment of High Throughput Sequencing Quality of Host DNA Enriched From Faeces: A Case From Captive Tiger.Molecular ecology resources · 2026Article
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- Development of High-Throughput Genomic Resources to Inform White-Tailed Deer Population and Disease Management.Molecular ecology resources · 2026Article
- Genomic Insights Into the Origin, Decline and Recovery of the Once Critically Endangered Iberian Lynx.Molecular ecology · 2025Review
- Development of a cost-effective, multifunctional SNP panel and analysis workflow for Wolf monitoring in Finland.Scientific reports · 2025Article
- Evolutionary history and seascape genomics of Harbour porpoises (Phocoena phocoena) across environmental gradients in the North Atlantic and adjacent waters.Molecular ecology resources · 2025Article
- Development of a SNP Panel for Geographic Assignment and Population Monitoring of Jaguars (Ecology and evolution · 2025Article
- Standardization of a High-Quality Methodological Framework for Long-Term Genetic Monitoring of the French Wolf Population.Ecology and evolution · 2025Article
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- Wildlife parasitology: sample collection and processing, diagnostic constraints, and methodological challenges in terrestrial carnivores.Parasites & vectors · 2024Review
- Species-specific SNP arrays for non-invasive genetic monitoring of a vulnerable bat.Scientific reports · 2024Article
- A reduced SNP panel optimised for non-invasive genetic assessment of a genetically impoverished conservation icon, the European bison.Scientific reports · 2024Article
- Evaluating the use of non-invasive hair sampling and ddRAD to characterize populations of endangered species: Application to a peripheral population of the European mink.Ecology and evolution · 2023Article
- Genome-wide variant analyses reveal new patterns of admixture and population structure in Australian dingoes.Molecular ecology · 2023Article
- A novel SNP assay reveals increased genetic variability and abundance following translocations to a remnant Allegheny woodrat population.BMC ecology and evolution · 2022Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Noninvasively collected samples are a common source of DNA in wildlife genetic studies. Currently, single nucleotide polymorphism (SNP) genotyping using microfluidic arrays is emerging as an easy-to-use and cost-effective methodology. Here we assessed the performance of microfluidic SNP arrays in genotyping noninvasive samples from grey wolves, European wildcats and brown bears, and we compared results with traditional microsatellite genotyping. We successfully SNP-genotyped 87%, 80% and 97% of the wolf, cat and bear samples, respectively. Genotype recovery was higher based on SNPs, while both marker types identified the same individuals and provided almost identical estimates of pairwise differentiation. We found that samples for which all SNP loci were scored had no disagreements across the three replicates (except one locus in a wolf sample). Thus, we argue that call rate (amplification success) can be used as a proxy for genotype quality, allowing the reduction of replication effort when call rate is high. Furthermore, we used cycle threshold values of real-time PCR to guide the choice of protocols for SNP amplification. Finally, we provide general guidelines for successful SNP genotyping of degraded DNA using microfluidic technology.
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