Evidence map›Paper›PMID 28882814›Full record

ArticleAmerican journal of physiology. Lung cellular and molecular physiology2018

Opsin 3 and 4 mediate light-induced pulmonary vasorelaxation that is potentiated by G protein-coupled receptor kinase 2 inhibition.

Sebastian Barreto Ortiz, Daijiro Hori, Yohei Nomura, Xin Yun, Haiyang Jiang, Hwanmee Yong, James Chen, Sam Paek, Deepesh Pandey, Gautam Sikka and 11 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in American journal of physiology. Lung cellular and molecular physiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.

0numbers the graph read from it
0cells of the map it votes in
44citing papers in PubMed
2.3field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

44 citing papers in PubMed, 62 citations in OpenAlex.

  1. Review
  2. Article
  3. Biomedical optics express · 2026
    Article
  4. Review
  5. Review
  6. Article
  7. Article
  8. Article
  9. β-Ionone facilitates ex vivo airway smooth muscle relaxation via extraocular opsin-3 light receptor activation.American journal of physiology. Lung cellular and molecular physiology · 2025
    Article
  10. Review
  11. Article
  12. Photobiomodulation therapy.JADA foundational science · 2025
    Review
  13. Light-sensitive CaProceedings of the National Academy of Sciences of the United States of America · 2024
    Article
  14. Melanopsin in the human and chicken choroid.Experimental eye research · 2024
    Article
  15. Sex differences and role of lysyl oxidase-like 2 in angiotensin II-induced hypertension in mice.American journal of physiology. Heart and circulatory physiology · 2024
    Article
  16. Article
  17. Article
  18. Review
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors at 2 institutions in 2 countries.

Sebastian Barreto OrtizDepartment of Anesthesiology and Critical Care Medicine, Johns Hopkins University , Baltimore, Maryland.ORCID 0000-0002-7093-4322
Daijiro HoriDepartment of Anesthesiology and Critical Care Medicine, Johns Hopkins University , Baltimore, Maryland.
Yohei NomuraDepartment of Anesthesiology and Critical Care Medicine, Johns Hopkins University , Baltimore, Maryland.
Xin YunDivision of Pulmonary and Critical Care Medicine, Johns Hopkins Asthma and Allergy Center, Johns Hopkins University , Baltimore, Maryland.
Haiyang JiangDivision of Pulmonary and Critical Care Medicine, Johns Hopkins Asthma and Allergy Center, Johns Hopkins University , Baltimore, Maryland.
Hwanmee YongDepartment of Environmental Health and Engineering, Johns Hopkins Bloomberg School of Public Health , Baltimore, Maryland.
James ChenDepartment of Biomedical Engineering, Johns Hopkins University , Baltimore, Maryland.
Sam PaekDepartment of Environmental Health and Engineering, Johns Hopkins Bloomberg School of Public Health , Baltimore, Maryland.
Deepesh PandeyDepartment of Anesthesiology and Critical Care Medicine, Johns Hopkins University , Baltimore, Maryland.
Gautam SikkaDepartment of Anesthesiology and Critical Care Medicine, Johns Hopkins University , Baltimore, Maryland.
Anil BhattaDepartment of Anesthesiology and Critical Care Medicine, Johns Hopkins University , Baltimore, Maryland.
Andrew GillardDepartment of Anesthesiology and Critical Care Medicine, Johns Hopkins University , Baltimore, Maryland.
Jochen SteppanDepartment of Anesthesiology and Critical Care Medicine, Johns Hopkins University , Baltimore, Maryland.
Jae Hyung KimDepartment of Anesthesiology and Critical Care Medicine, Johns Hopkins University , Baltimore, Maryland.
Hideo AdachiDepartment of Cardiovascular Surgery, Saitama Medical Center, Jichi Medical University, Shimotsuke, Japan.
Viachaslau M BarodkaDepartment of Anesthesiology and Critical Care Medicine, Johns Hopkins University , Baltimore, Maryland.
Lewis RomerDepartment of Anesthesiology and Critical Care Medicine, Johns Hopkins University , Baltimore, Maryland.
Steven S AnDepartment of Environmental Health and Engineering, Johns Hopkins Bloomberg School of Public Health , Baltimore, Maryland.
Larissa A ShimodaDivision of Pulmonary and Critical Care Medicine, Johns Hopkins Asthma and Allergy Center, Johns Hopkins University , Baltimore, Maryland.
Lakshmi SanthanamDepartment of Anesthesiology and Critical Care Medicine, Johns Hopkins University , Baltimore, Maryland.
Dan E BerkowitzDepartment of Anesthesiology and Critical Care Medicine, Johns Hopkins University , Baltimore, Maryland.
Johns Hopkins University · USJichi Medical University · JP

Funding

Project 4 - OGR1- and TSPO- dependent mechanisms mediated by benzodiazepines affecting ASM contractionP01HL114471 · NHLBI · UNIVERSITY OF PENNSYLVANIA · PI BENOVIC, JEFFREY L · 2013 to 2023
$23.9M
Nrf2 as a critical determinant of smooth muscle functionR01HL107361 · NHLBI · JOHNS HOPKINS UNIVERSITY · PI AN, STEVEN S · 2011 to 2015
$2.0M
Non-Visual Opsins & Vasoregulation: Implications for Vascular TherapyR01HL124213 · NHLBI · JOHNS HOPKINS UNIVERSITY · PI BERKOWITZ, DAN E · 2015 to 2018
$1.6M
Zeiss LSM780 Confocal Microscope for a Core FacilityS10OD016374 · OD · JOHNS HOPKINS UNIVERSITY · PI KUO, SCOT CHARLES · 2013 to 2013
$584k
NHLBI NIH HHS P01 HL114471NHLBI NIH HHS R01 HL107361NHLBI NIH HHS R01 HL124213NIH HHS S10 OD016374
6 · The paper itself

Abstract

We recently demonstrated that blue light induces vasorelaxation in the systemic mouse circulation, a phenomenon mediated by the nonvisual G protein-coupled receptor melanopsin (Opsin 4; Opn4). Here we tested the hypothesis that nonvisual opsins mediate photorelaxation in the pulmonary circulation. We discovered Opsin 3 (Opn3), Opn4, and G protein-coupled receptor kinase 2 (GRK2) in rat pulmonary arteries (PAs) and in pulmonary arterial smooth muscle cells (PASMCs), where the opsins interact directly with GRK2, as demonstrated with a proximity ligation assay. Light elicited an intensity-dependent relaxation of PAs preconstricted with phenylephrine (PE), with a maximum response between 400 and 460 nm (blue light). Wavelength-specific photorelaxation was attenuated in PAs from Opn4

Indexed as

PhototherapyAnimalsCells, CulturedG-Protein-Coupled Receptor Kinase 2Hypertension, PulmonaryHypoxiaLightMaleMelanopsinMiceMice, Inbred C57BLMice, KnockoutMuscle, Smooth, VascularNitric OxidePulmonary ArteryRatsG-Protein-Coupled Receptor Kinase 2MelanopsinNitric OxideOPN3 protein, mouseRod OpsinsSoluble Guanylyl CyclaseGRK2opsinsphotorelaxationpulmonary artery smooth musclepulmonary hypertension

Identifiers

PMID28882814
PMCPMC6335007
OpenAlexW2752918895

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.