ArticleBMC complementary and alternative medicine2017
Is cytotoxicity a determinant of the different in vitro and in vivo effects of bioactives?
Article in BMC complementary and alternative medicine, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
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- Plant derived tannic acid-based tissue adhesives: From mechanism to advanced design.Acta biomaterialia · 2026Review
- Investigation of the effects of a Kiperin Bromelain & Papain complex in normal colon, mammary, and skin cells.Scientific reports · 2026Article
- Preventive and inhibitory effects ofBiochemistry and biophysics reports · 2026Article
- Bioactive galloylquinic acids from Copaifera lucens as dual inhibitors of SARS-CoV-2 Spike and RdRp proteins.Scientific reports · 2026Article
- Evaluation of physcion isolated from endophytic Aspergillus fumigatus ASH3 as an antiviral agent against coxsackievirus B4 and herpes simplex virus type 1: in silico-supported in vitro study.Folia microbiologica · 2026Article
- Optimizing INFOGEST Digest Conditioning for Reliable In Vitro Assessment of Nutrient Bioavailability Using Caco-2 Cell Models.Nutrients · 2026Article
- Chemical Profile and In Vitro Protective Effects ofPlants (Basel, Switzerland) · 2025Article
- Synergistic anti-malarial, cytotoxic, and antioxidant activities of trimetallic (Au-Pt-Pd) and bimetallic (Au-Pt) nanofluids: in vitro and computational insights.BMC pharmacology & toxicology · 2025Article
- Cytotoxicity, pro-inflammatory cytokines gene expression and antioxidant activity ofNatural product research · 2025Article
- In vitro evaluation of cytotoxicity and release kinetics of green-synthesisedCytotechnology · 2025Article
- An ex vivo Approach in European Seabass Leucocytes Supports the in vitro Regulation by Postbiotics of Aip56 Gene Expression of Photobacterium damselae subsp. piscicida.Probiotics and antimicrobial proteins · 2025Article
- Olive Mill Wastewater Extract: In Vitro Genotoxicity/Antigenotoxicity Assessment on HepaRG Cells.International journal of environmental research and public health · 2024Article
- Evaluation of the Differential Postbiotic Potential of Shewanella putrefaciens Pdp11 Cultured in Several Growing Conditions.Marine biotechnology (New York, N.Y.) · 2024Article
- A Curcumin-Based Oral Gel Has Potential Protective Efficacy against Oral Mucositis: In Vitro Study.Journal of personalized medicine · 2023Article
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- In vitro toxicological assessment of PhSeZnCl in human liver cells.Toxicological research · 2023Article
- Cytotoxicity and Genotoxicity ofInternational journal of environmental research and public health · 2022Article
- Mass Spectrometric Identification ofMolecules (Basel, Switzerland) · 2022Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundFoodstuffs of both plant and animal origin contain a wide range of bioactive compounds. Although human intervention studies are mandatory to assess the health effects of bioactives, the in vitro approach is often used to select the most promising molecules to be studied in vivo. To avoid misleading results, concentration and chemical form, exposure time, and potential cytotoxicity of the tested bioactives should be carefully set prior to any other experiments.
methodsIn this study the possible cytotoxicity of different bioactives (docosahexaenoic acid, propionate, cyanidin-3-O-glucoside, protocatechuic acid), was investigated in HepG2 cells using different methods. Bioactives were supplemented to cells at different concentrations within the physiological range in human blood, alone or in combination, considering two different exposure times.
resultsReported data clearly evidence that in vitro cytotoxicity is tightly related to the exposure time, and it varies among bioactives, which could exert a cytotoxic effect even at a concentration within the in vivo physiological blood concentration range. Furthermore, co-supplementation of different bioactives can increase the cytotoxic effect.
conclusionsOur results underline the importance of in vitro cytotoxicity screening that should be considered mandatory before performing studies aimed to evaluate the effect of bioactives on other cellular parameters. Although this study is far from the demonstration of a toxic effect of the tested bioactives when administered to humans, it represents a starting point for future research aimed at verifying the existence of a potential hazard due to the wide use of high doses of multiple bioactives.
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