Evidence map›Paper›PMID 28880934›Full record

ArticlePloS one2017

DNA methylation patterns and gene expression associated with litter size in Berkshire pig placenta.

Jung Hye Hwang, Sang Mi An, Seulgi Kwon, Da Hye Park, Tae Wan Kim, Deok Gyeong Kang, Go Eun Yu, Il-Suk Kim, Hwa Chun Park, Jeongim Ha and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
1.1field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 27 citations in OpenAlex.

  1. Article
  2. Placental adaptation to maternal environment in domestic animals.The Journal of reproduction and development · 2026
    Review
  3. Article
  4. Review
  5. Effect of SNPs on Litter Size in Swine.Current issues in molecular biology · 2024
    Article
  6. Review
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Review
  15. Article
  16. Article
  17. Article
  18. DNA methylation pattern of the goatArchives animal breeding · 2019
    Article
  19. Hypomethylation in the promoter region ofArchives animal breeding · 2019
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 1 institution in 1 country.

Jung Hye HwangSwine Science and Technology Center, Gyeongnam National University of Science & Technology, Jinju, South Korea.
Sang Mi AnSwine Science and Technology Center, Gyeongnam National University of Science & Technology, Jinju, South Korea.ORCID http://orcid.org/0000-0002-2617-2025
Seulgi KwonSwine Science and Technology Center, Gyeongnam National University of Science & Technology, Jinju, South Korea.
Da Hye ParkSwine Science and Technology Center, Gyeongnam National University of Science & Technology, Jinju, South Korea.
Tae Wan KimSwine Science and Technology Center, Gyeongnam National University of Science & Technology, Jinju, South Korea.
Deok Gyeong KangSwine Science and Technology Center, Gyeongnam National University of Science & Technology, Jinju, South Korea.
Go Eun YuSwine Science and Technology Center, Gyeongnam National University of Science & Technology, Jinju, South Korea.
Il-Suk KimDepartment of Animal Resource Technology, Gyeongnam National University of Science & Technology, Jinju, South Korea.
Hwa Chun ParkDasan Pig Breeding Co., Namwon, South Korea.
Jeongim HaSwine Science and Technology Center, Gyeongnam National University of Science & Technology, Jinju, South Korea.
Chul Wook KimSwine Science and Technology Center, Gyeongnam National University of Science & Technology, Jinju, South Korea.
Gyeongnam National University of Science and Technology · KR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Increasing litter size is of great interest to the pig industry. DNA methylation is an important epigenetic modification that regulates gene expression, resulting in livestock phenotypes such as disease resistance, milk production, and reproduction. We classified Berkshire pigs into two groups according to litter size and estimated breeding value: smaller (SLG) and larger (LLG) litter size groups. Genome-wide DNA methylation and gene expression were analyzed using placenta genomic DNA and RNA to identify differentially methylated regions (DMRs) and differentially expressed genes (DEGs) associated with litter size. The methylation levels of CpG dinucleotides in different genomic regions were noticeably different between the groups, while global methylation pattern was similar, and excluding intergenic regions they were found the most frequently in gene body regions. Next, we analyzed RNA-Seq data to identify DEGs between the SLG and LLG groups. A total of 1591 DEGs were identified: 567 were downregulated and 1024 were upregulated in LLG compared to SLG. To identify genes that simultaneously exhibited changes in DNA methylation and mRNA expression, we integrated and analyzed the data from bisulfite-Seq and RNA-Seq. Nine DEGs positioned in DMRs were found. The expression of only three of these genes (PRKG2, CLCA4, and PCK1) was verified by RT-qPCR. Furthermore, we observed the same methylation patterns in blood samples as in the placental tissues by PCR-based methylation analysis. Together, these results provide useful data regarding potential epigenetic markers for selecting hyperprolific sows.

Indexed as

AnimalsCpG IslandsDNA MethylationEpigenesis, GeneticFemaleGene ExpressionGene Expression ProfilingLitter SizePlacentaPregnancySwine

Identifiers

PMID28880934
PMCPMC5589248
OpenAlexW2750777289

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.