ArticleNature protocols2017
Live-cell confocal microscopy and quantitative 4D image analysis of anchor-cell invasion through the basement membrane in Caenorhabditis elegans.
Article in Nature protocols, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Specialized high-capacity mitochondria fuel cell invasion.Current biology : CB · 2026Article
- Programmed mitophagy at the oocyte-to-zygote transition promotes lineage endurance.Nature cell biology · 2026Article
- Knockdown-Induced Fasting Phenotypes in Flatworms: Insights into Underlying Mechanisms of Feeding Behavior.International journal of molecular sciences · 2025Article
- A collagen IV fluorophore knock-in toolkit reveals trimer diversity in C. elegans basement membranes.The Journal of cell biology · 2025Article
- Shaping epithelial tissues by stem cell mechanics in development and cancer.Nature reviews. Molecular cell biology · 2025Review
- De novo lipid synthesis and polarized prenylation drive cell invasion through basement membrane.The Journal of cell biology · 2024Article
- The C. elegans anchor cell: A model to elucidate mechanisms underlying invasion through basement membrane.Seminars in cell & developmental biology · 2024Review
- Cell cycle perturbation uncouples mitotic progression and invasive behavior in a post-mitotic cell.bioRxiv : the preprint server for biology · 2024Article
- An mTurq2-Col4a1 mouse model allows for live visualization of mammalian basement membrane development.The Journal of cell biology · 2024Article
- Article
- A Window into Mammalian Basement Membrane Development: Insights from thebioRxiv : the preprint server for biology · 2023Article
- Reciprocal discoidin domain receptor signaling strengthens integrin adhesion to connect adjacent tissues.eLife · 2023Article
- Reciprocal discoidin domain receptor signaling strengthens integrin adhesion to connect adjacent tissues.bioRxiv : the preprint server for biology · 2023Article
- Hemicentin-mediated type IV collagen assembly strengthens juxtaposed basement membrane linkage.The Journal of cell biology · 2023Article
- Differential production rates of cytosolic and transmembrane GFP reporters inmicroPublication biology · 2023Article
- Visualizing cytoplasmic ATP inSTAR protocols · 2022Article
- Localized glucose import, glycolytic processing, and mitochondria generate a focused ATP burst to power basement-membrane invasion.Developmental cell · 2022Article
- A light sheet fluorescence microscopy protocol forFrontiers in cell and developmental biology · 2022Article
- Reciprocal EGFR signaling in the anchor cell ensures precise inter-organ connection during Caenorhabditis elegans vulval morphogenesis.Development (Cambridge, England) · 2022Article
- Quantitative Approaches to Study Retinal Neurogenesis.Biomedicines · 2021Review
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Cell invasion through basement membrane (BM) barriers is crucial in development, leukocyte trafficking and the spread of cancer. The mechanisms that direct invasion, despite their importance in normal and disease states, are poorly understood, largely because of the inability to visualize dynamic cell-BM interactions in vivo. This protocol describes multichannel time-lapse confocal imaging of anchor-cell invasion in live Caenorhabditis elegans. Methods presented include outline-slide preparation and worm growth synchronization (15 min), mounting (20 min), image acquisition (20-180 min), image processing (20 min) and quantitative analysis (variable timing). The acquired images enable direct measurement of invasive dynamics including formation of invadopodia and cell-membrane protrusions, and removal of BM. This protocol can be combined with genetic analysis, molecular-activity probes and optogenetic approaches to uncover the molecular mechanisms underlying cell invasion. These methods can also be readily adapted by any worm laboratory for real-time analysis of cell migration, BM turnover and cell-membrane dynamics.
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Registered trials
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