ArticleNature protocols2017
Preparation of biogenic gas vesicle nanostructures for use as contrast agents for ultrasound and MRI.
Article in Nature protocols, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 92 papers.
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Who cites it
92 citing papers in PubMed, 178 citations in OpenAlex.
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- Ultrasound-Mediated OFF-to-ON Switching of Bone Regeneration via a Bioorthogonal Cell-Capture Hydrogel, Enabling Spatiotemporal Control and Monitoring.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Multiplexed optoacoustic tracking and magnetic actuation of labeled blood cells in living mice.Science advances · 2026Article
- Cavitation behaviors of genetically engineered bacterial protein nanoparticles induced by pulsed ultrasound.Ultrasonics sonochemistry · 2026Article
- Real-time volumetric imaging of cells and molecules in deep tissues with Takoyaki ultrasound.Nature communications · 2026Article
- Evaluation of Resorcinarene Trimer Methanesulfonate Aggregation in the Presence of Inorganic Salts and Biomolecules in Aqueous Solutions Using UV-vis Spectroscopy and Electrophoresis.Chemphyschem : a European journal of chemical physics and physical chemistry · 2026Article
- Screening and Evaluation of Chassis Cells for Heterologous Biosynthesis of Gas Vesicles as Ultrasound Contrast Agents.Marine drugs · 2026Article
- Ultrasound-driven mechanophore activation in living plants.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Enzymatic microbubble robots.Nature nanotechnology · 2026Article
- Temporal gene regulation enables controlled expression of gas vesicles and preserves bacterial viability.Nature communications · 2025Article
- Enhanced Ultrasound Image Formation With Computationally Efficient Cross-Angular Delay Multiply and Sum Beamforming.Ultrasound in medicine & biology · 2025Article
- The Binding Affinities of Serum Proteins to Nanoparticles.Journal of the American Chemical Society · 2025Article
- Ultrasound-Mediated Membrane Modulation for Biomedical Applications.Nanomaterials (Basel, Switzerland) · 2025Review
- Acoustic percolation switches enable targeted drug delivery controlled by diagnostic ultrasound.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Excavation of acoustic nanostructures biosynthesis gene clusters by combinatorial strategy.Advanced biotechnology · 2025Article
- PEGylated gas vesicles: a promising novel ultrasound contrast agent for diagnosis and guiding radiofrequency ablation of liver tumor.Journal of nanobiotechnology · 2025Article
- Imaging-guided deep tissue in vivo sound printing.Science (New York, N.Y.) · 2025Article
- Nonlinear sound-sheet microscopy: Imaging opaque organs at the capillary and cellular scale.Science (New York, N.Y.) · 2025Article
- Surface-engineered bio-manufactured gas vesicles for multimodal imaging of glioma.Journal of nanobiotechnology · 2025Article
- UltrasensitiveAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
32 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
15 authors at 3 institutions in 3 countries.
Funding
Abstract
Gas vesicles (GVs) are a unique class of gas-filled protein nanostructures that are detectable at subnanomolar concentrations and whose physical properties allow them to serve as highly sensitive imaging agents for ultrasound and MRI. Here we provide a protocol for isolating GVs from native and heterologous host organisms, functionalizing these nanostructures with moieties for targeting and fluorescence, characterizing their biophysical properties and imaging them using ultrasound and MRI. GVs can be isolated from natural cyanobacterial and haloarchaeal host organisms or from Escherichia coli expressing a heterologous GV gene cluster and purified using buoyancy-assisted techniques. They can then be modified by replacing surface-bound proteins with engineered, heterologously expressed variants or through chemical conjugation, resulting in altered mechanical, surface and targeting properties. Pressurized absorbance spectroscopy is used to characterize their mechanical properties, whereas dynamic light scattering (DLS)and transmission electron microscopy (TEM) are used to determine nanoparticle size and morphology, respectively. GVs can then be imaged with ultrasound in vitro and in vivo using pulse sequences optimized for their detection versus background. They can also be imaged with hyperpolarized xenon MRI using chemical exchange saturation transfer between GV-bound and dissolved xenon-a technique currently implemented in vitro. Taking 3-8 d to prepare, these genetically encodable nanostructures enable multimodal, noninvasive biological imaging with high sensitivity and potential for molecular targeting.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.