Evidence map›Paper›PMID 28879199›Full record

ArticleMolecular neuropsychiatry2017

Low-Density Neuronal Cultures from Human Induced Pluripotent Stem Cells.

Peter Dimitrion, Yun Zhi, Dennis Clayton, Gerard L Apodaca, Madeleine R Wilcox, Jon W Johnson, Vishwajit Nimgaonkar, Leonardo D'Aiuto

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular neuropsychiatry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Childhood-Onset Schizophrenia: Insights from Induced Pluripotent Stem Cells.International journal of molecular sciences · 2018
    Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Peter DimitrionDepartment of Psychiatry, Western Psychiatric Institute and Clinic, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA.
Yun ZhiDepartment of Pharmacology and Pharmaceutical Sciences, School of Medicine, Tsinghua University, Beijing, China.
Dennis ClaytonDivision of Renal-Electrolyte, Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
Gerard L ApodacaDivision of Renal-Electrolyte, Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
Madeleine R WilcoxDepartment of Neuroscience and Center for Neuroscience, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
Jon W JohnsonDepartment of Neuroscience and Center for Neuroscience, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
Vishwajit NimgaonkarDepartment of Psychiatry, Western Psychiatric Institute and Clinic, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA.
Leonardo D'AiutoDepartment of Psychiatry, Western Psychiatric Institute and Clinic, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA.

Funding

Single Nephron and MetabolomicsP30DK079307 · NIDDK · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI KLEYMAN, THOMAS R · 2008 to 2022
$13.4M
NEUROBEHAVIORAL STUDY OF SCHIZOPHRENIAR01MH042191 · NIMH · UNIVERSITY OF PENNSYLVANIA · PI GUR, RAQUEL E · 1987 to 2018
$7.1M
A Neurobehavioral Family Study of SchizophreniaR01MH063480 · NIMH · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI NIMGAONKAR, VISHWAJIT LAXMIKANT · 2001 to 2018
$6.1M
Permeation, Block, and Gating of NMDA ReceptorsR01MH045817 · NIMH · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI JOHNSON, JON W. · 1997 to 2017
$5.0M
NIDDK NIH HHS P30 DK079307NIMH NIH HHS R01 MH042191NIMH NIH HHS R01 MH045817NIMH NIH HHS R01 MH063480
6 · The paper itself

Abstract

Induced pluripotent stem cell (iPSC)-based technologies offer an unprecedented possibility to investigate defects occurring during neuronal differentiation in neuropsychiatric and neurodevelopmental disorders, but the density and intricacy of intercellular connections in neuronal cultures challenge currently available analytic methods. Low-density neuronal cultures facilitate the morphometric and functional analysis of neurons. We describe a differentiation protocol to generate low-density neuronal cultures (∼2,500 neurons/cm

Indexed as

Human induced pluripotent stem cellsLow-density neuronal cultures

Identifiers

PMID28879199
PMCPMC5582512

What OpenQuestion holds

Textmetadata
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.