ArticleScientific reports2017
Identification of oriT and a recombination hot spot in the IncA/C plasmid backbone.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 22 citations in OpenAlex.
- Conjugative transfer inhibition of IncA and IncC plasmids by SGI1-like elements via relaxosome assembly interference.Nucleic acids research · 2026Article
- Bioengineering Interventions to Enhance the Capacity of the Gut Microbiota in Controlling Food Allergies.Life (Basel, Switzerland) · 2026Review
- Towards quantifying plasmid similarity.Microbial genomics · 2024Review
- Staphylococcus equorum plasmid pKS1030-3 encodes auxiliary biofilm formation and trans-acting gene mobilization systems.Scientific reports · 2023Article
- Conjugative IncC Plasmid Entry Triggers the SOS Response and Promotes Effective Transfer of the Integrative Antibiotic Resistance Element SGI1.Microbiology spectrum · 2023Article
- Development of an antimicrobial resistance plasmid transfer gene database for enteric bacteria.Frontiers in bioinformatics · 2023Article
- Wastewater bacteria remediating the pharmaceutical metformin: Genomes, plasmids and products.Frontiers in bioengineering and biotechnology · 2022Article
- Comparative genomics reveals new insights into the evolution of the IncA and IncC family of plasmids.Frontiers in microbiology · 2022Article
- Salmonella Genomic Island 1 requires a self-encoded small RNA for mobilization.Molecular microbiology · 2021Article
- Crucial role of Salmonella genomic island 1 master activator in the parasitism of IncC plasmids.Nucleic acids research · 2021Article
- Comprehensive analysis of IncC plasmid conjugation identifies a crucial role for the transcriptional regulator AcaB.Nature microbiology · 2020Article
- Article
- Transferable Plasmids ofFrontiers in microbiology · 2020Review
- Identification and Characterization ofFrontiers in microbiology · 2019Article
- Relaxase MobM Induces a Molecular Switch at Its Cognate Origin of Transfer.Frontiers in molecular biosciences · 2018Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dissemination of multiresistance has been accelerating among pathogenic bacteria in recent decades. The broad host-range conjugative plasmids of the IncA/C family are effective vehicles of resistance determinants in Gram-negative bacteria. Although more than 150 family members have been sequenced to date, their conjugation system and other functions encoded by the conserved plasmid backbone have been poorly characterized. The key cis-acting locus, the origin of transfer (oriT), has not yet been unambiguously identified. We present evidence that IncA/C plasmids have a single oriT locus immediately upstream of the mobI gene encoding an indispensable transfer factor. The fully active oriT spans ca. 150-bp AT-rich region overlapping the promoters of mobI and contains multiple inverted and direct repeats. Within this region, the core domain of oriT with reduced but detectable transfer activity was confined to a 70-bp segment containing two inverted repeats and one copy of a 14-bp direct repeat. In addition to oriT, a second locus consisting of a 14-bp imperfect inverted repeat was also identified, which mimicked the function of oriT but which was found to be a recombination site. Recombination between two identical copies of these sites is RecA-independent, requires a plasmid-encoded recombinase and resembles the functioning of dimer-resolution systems.
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