ArticleScientific reports2017
Culture-independent metagenomics supports discovery of uncultivable bacteria within the genus Chlamydia.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Ultrastructural Insights Into a Candidatus Parilichlamydia sp. Infection of Gill Goblet Cells in Greater Amberjack.Journal of fish diseases · 2025Article
- Pangenome-Wide Association Study in theInternational journal of molecular sciences · 2024Article
- Chlamydiosis in Animals.Animals : an open access journal from MDPI · 2024Review
- Seroprevalence and Risk Factors ofVector borne and zoonotic diseases (Larchmont, N.Y.) · 2024Article
- Zoonotic and other veterinary chlamydiae - an update, the role of the plasmid and plasmid-mediated transformation.Pathogens and disease · 2024Review
- Whole Genome Sequencing and Comparative Genomic Analysis ofPathogens (Basel, Switzerland) · 2023Article
- Review
- Gene gain facilitated endosymbiotic evolution of Chlamydiae.Nature microbiology · 2023Article
- Genomic diversity and biosynthetic capabilities of sponge-associated chlamydiae.The ISME journal · 2022Article
- An in silico analysis of rpoB mutations to affect Chlamydia trachomatis sensitivity to rifamycin.Journal, genetic engineering & biotechnology · 2022Article
- Occurrence of Chlamydiae in Corvids in Northeast Italy.Animals : an open access journal from MDPI · 2022Article
- Detection of a Novel Chlamydia Species in Invasive Turtles.Animals : an open access journal from MDPI · 2022Article
- Whole Genome Sequencing and Comparative Genome Analyses ofPathogens (Basel, Switzerland) · 2021Article
- Metagenome-assembled genome of a Chitinophaga sp. and its potential in plant biomass degradation, as well of affiliated Pandoraea and Labrys species.World journal of microbiology & biotechnology · 2021Article
- Seroprevalence of Chlamydia abortus infection in yak (Bos grunniens) in Tibet, China.Irish veterinary journal · 2021Article
- Draft Genome Sequences of Avian Chlamydia abortus Genotype G2 Strain 15-49d3, Isolated from Mallard, and Genotype 1V Strain 15-58d44, Isolated from Magpie in Poland.Microbiology resource announcements · 2021Article
- Whole genome de novo sequencing and comparative genomic analyses suggests that Chlamydia psittaci strain 84/2334 should be reclassified as Chlamydia abortus species.BMC genomics · 2021Article
- Article
- Chlamydiae - What's New?Journal of veterinary research · 2020Article
- Chronic wasting associated with Chlamydia pneumoniae in three ex situ breeding facilities for tropical frogs.Antonie van Leeuwenhoek · 2020Article
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Authors and funding
4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Advances in culture-independent methods have meant that we can more readily detect and diagnose emerging infectious disease threats in humans and animals. Metagenomics is fast becoming a popular tool for detection and characterisation of novel bacterial pathogens in their environment, and is particularly useful for obligate intracellular bacteria such as Chlamydiae that require labour-intensive culturing. We have used this tool to investigate the microbial metagenomes of Chlamydia-positive cloaca and choana samples from snakes. The microbial complexity within these anatomical sites meant that despite previous detection of chlamydial 16S rRNA sequences by single-gene broad-range PCR, only a chlamydial plasmid could be detected in all samples, and a chlamydial chromosome in one sample. Comparative genomic analysis of the latter revealed it represented a novel taxon, Ca. Chlamydia corallus, with genetic differences in regards to purine and pyrimidine metabolism. Utilising statistical methods to relate plasmid phylogeny to the phylogeny of chromosomal sequences showed that the samples also contain additional novel strains of Ca. C. corallus and two putative novel species in the genus Chlamydia. This study highlights the value of metagenomics methods for rapid novel bacterial discovery and the insights it can provide into the biology of uncultivable intracellular bacteria such as Chlamydiae.
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