ArticleScientific reports2017
A fluorescence-based reporter for monitoring expression of mycobacterial cytochrome bd in response to antibacterials and during infection.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 20 citations in OpenAlex.
- The role of cytochrome oxidases in bacterial virulence.Infection and immunity · 2026Review
- Usnic acid impacts energy production and iron metabolism inmSystems · 2025Article
- Identification of genes associated with persistence inFrontiers in microbiology · 2024Article
- Chemical approaches to unraveling the biology of mycobacteria.Cell chemical biology · 2023Review
- Phenotypic adaptation ofFrontiers in cellular and infection microbiology · 2022Review
- Mechanistic and structural diversity between cytochromeProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Bacterial Oxidases of the CytochromeAntioxidants & redox signaling · 2021Review
- Heterologous Expression ofAntimicrobial agents and chemotherapy · 2021Article
- Article
- Anti-tuberculosis effect of isoniazid scales accurately from zebrafish to humans.British journal of pharmacology · 2020Article
- Induction of theFrontiers in microbiology · 2020Article
- Characterization and Transcriptome Studies of Autoinducer Synthase Gene from Multidrug ResistantGenes · 2019Article
- Isoniazid Bactericidal Activity Involves Electron Transport Chain Perturbation.Antimicrobial agents and chemotherapy · 2019Article
- Fluorescent Mycobacterium tuberculosis reporters: illuminating host-pathogen interactions.Pathogens and disease · 2018Review
- Bioenergetics of Mycobacterium: An Emerging Landscape for Drug Discovery.Pathogens (Basel, Switzerland) · 2018Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytochrome bd is a component of the oxidative phosphorylation pathway in many Gram-positive and Gram-negative bacteria. Next to its role as a terminal oxidase in the respiratory chain this enzyme plays an important role as a survival factor in the bacterial stress response. In Mycobacterium tuberculosis and related mycobacterial strains, cytochrome bd is an important component of the defense system against antibacterial drugs. In this report we describe and evaluate an mCherry-based fluorescent reporter for detection of cytochrome bd expression in Mycobacterium marinum. Cytochrome bd was induced by mycolic acid biosynthesis inhibitors such as isoniazid and most prominently by drugs targeting oxidative phosphorylation. We observed no induction by inhibitors of protein-, DNA- or RNA-synthesis. The constructed expression reporter was suitable for monitoring mycobacterial cytochrome bd expression during mouse macrophage infection and in a zebrafish embryo infection model when using Mycobacterium marinum. Interestingly, in both these infection models cytochrome bd levels were considerably higher than during in vitro culturing of M. marinum. The expression reporter described here can be a valuable tool for elucidating the role of cytochrome bd as a survival factor.
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