Evidence map›Paper›PMID 28878000›Full record

ArticleThe Journal of experimental medicine2017

Dissecting hematopoietic and renal cell heterogeneity in adult zebrafish at single-cell resolution using RNA sequencing.

Qin Tang, Sowmya Iyer, Riadh Lobbardi, John C Moore, Huidong Chen, Caleb Lareau, Christine Hebert, McKenzie L Shaw, Cyril Neftel, Mario L Suva and 6 more

Open access · bronzeAbstract read
In one paragraph

Article in The Journal of experimental medicine, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 120 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
120citing papers in PubMed, 1 pooled it
7.2field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

120 citing papers in PubMed, 1 synthesis or guideline pooled it, 221 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Lessons from the Axolotl: De Novo Nephron Formation after Nephrectomy.Journal of the American Society of Nephrology : JASN · 2026
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  8. Single-cell atlas of grass carp (Zoological research · 2025
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  11. HemAtlas: A Multi-omics Hematopoiesis Database.Genomics, proteomics & bioinformatics · 2025
    Article
  12. Review
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Zebrafish (International journal of molecular sciences · 2024
    Review
  20. Article

60 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

16 authors at 5 institutions in 2 countries.

Qin TangMolecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA.ORCID http://orcid.org/0000-0002-9487-570X
Sowmya IyerMolecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA.
Riadh LobbardiMolecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA.
John C MooreMolecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA.ORCID http://orcid.org/0000-0003-0242-3894
Huidong ChenDepartment of Biostatistics, T.H. Chan School of Public Health, Harvard University, Boston, MA.
Caleb LareauCenter for Computational and Integrative Biology, Massachusetts General Hospital, Charlestown, MA.ORCID http://orcid.org/0000-0003-4179-4807
Christine HebertMolecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA.
McKenzie L ShawMolecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA.
Cyril NeftelMolecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA.
Mario L SuvaMolecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA.
Craig J CeolDepartment of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, Worcester, MA.ORCID http://orcid.org/0000-0002-7188-7580
Andre BernardsCenter for Cancer Research, Massachusetts General Hospital, Charlestown, MA.
Martin AryeeCenter for Computational and Integrative Biology, Massachusetts General Hospital, Charlestown, MA.
Luca PinelloMolecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA.ORCID http://orcid.org/0000-0003-1109-3823
Iain A DrummondDivision of Nephrology, Department of Medicine, Massachusetts General Hospital, Boston, MA.
David M LangenauMolecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA dlangenau@mgh.harvard.edu.ORCID http://orcid.org/0000-0001-6664-8318
Harvard University · USBroad Institute · USMassachusetts General Hospital · USTongji University · CNUniversity of Massachusetts Chan Medical School · US

Funding

GENETIC ANALYSIS OF ZEBRAFISH KIDNEY DEVELOPMENTR01DK053093 · NIDDK · MASSACHUSETTS GENERAL HOSPITAL · PI DRUMMOND, IAIN A. · 1997 to 2018
$5.2M
Immune Compromises Zebrafish for Cell TransplantationR24OD016761 · OD · MASSACHUSETTS GENERAL HOSPITAL · PI LANGENAU, DAVID MICHAEL · 2013 to 2021
$5.2M
Use of comparative genomics to identify novel regulators of melanoma progressionR01AR063850 · NIAMS · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI CEOL, CRAIG JOSEPH · 2013 to 2017
$1.8M
Mechanisms of tubule interconnectionUH3DK107372 · NIDDK · MOUNT DESERT ISLAND BIOLOGICAL LAB · PI DRUMMOND, IAIN A. · 2017 to 2019
$1.3M
NIAMS NIH HHS R01 AR063850NIDDK NIH HHS R01 DK053093NIDDK NIH HHS UH3 DK107372NIH HHS R24 OD016761
6 · The paper itself

Abstract

Recent advances in single-cell, transcriptomic profiling have provided unprecedented access to investigate cell heterogeneity during tissue and organ development. In this study, we used massively parallel, single-cell RNA sequencing to define cell heterogeneity within the zebrafish kidney marrow, constructing a comprehensive molecular atlas of definitive hematopoiesis and functionally distinct renal cells found in adult zebrafish. Because our method analyzed blood and kidney cells in an unbiased manner, our approach was useful in characterizing immune-cell deficiencies within DNA-protein kinase catalytic subunit (

Indexed as

AnimalsAnimals, Genetically ModifiedCell LineageGene Expression ProfilingHematopoiesis, ExtramedullaryHematopoietic Stem CellsKidneyRNASequence Analysis, RNAZebrafishRNA

Identifiers

PMID28878000
PMCPMC5626406
OpenAlexW2753518299

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.