Evidence map›Paper›PMID 28877474›Full record

ArticleCell reports2017

Long Noncoding RNA PURPL Suppresses Basal p53 Levels and Promotes Tumorigenicity in Colorectal Cancer.

Xiao Ling Li, Murugan Subramanian, Matthew F Jones, Ritu Chaudhary, Deepak K Singh, Xinying Zong, Berkley Gryder, Sivasish Sindri, Min Mo, Aaron Schetter and 19 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 115 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
115citing papers in PubMed, 1 pooled it
5.0field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

115 citing papers in PubMed, 1 synthesis or guideline pooled it, 154 citations in OpenAlex.

  1. Pooled it
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  4. Intron Retention Controls Localization of lncRNAsbioRxiv : the preprint server for biology · 2026
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  7. Review
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  16. Article
  17. Article
  18. Functional Bidirectionality of ERV-Derived Long Non-Coding RNAs in Humans.International journal of molecular sciences · 2024
    Review
  19. eLife · 2024
    Article
  20. Article

55 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

29 authors at 10 institutions in 2 countries.

Xiao Ling LiRegulatory RNAs and Cancer Section, Genetics Branch, Center for Cancer Research (CCR), National Cancer Institute (NCI), NIH, Bethesda, MD 20892, USA.
Murugan SubramanianRegulatory RNAs and Cancer Section, Genetics Branch, Center for Cancer Research (CCR), National Cancer Institute (NCI), NIH, Bethesda, MD 20892, USA.
Matthew F JonesRegulatory RNAs and Cancer Section, Genetics Branch, Center for Cancer Research (CCR), National Cancer Institute (NCI), NIH, Bethesda, MD 20892, USA.
Ritu ChaudharyRegulatory RNAs and Cancer Section, Genetics Branch, Center for Cancer Research (CCR), National Cancer Institute (NCI), NIH, Bethesda, MD 20892, USA.
Deepak K SinghDepartment of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Xinying ZongDepartment of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Berkley GryderOncogenomics Section, Genetics Branch, CCR, NCI, NIH, Bethesda, MD 20892, USA.
Sivasish SindriOncogenomics Section, Genetics Branch, CCR, NCI, NIH, Bethesda, MD 20892, USA.
Min MoLaboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA.
Aaron SchetterMolecular Genetics and Carcinogenesis Section, Laboratory of Human Carcinogenesis, CCR, NCI, NIH, Bethesda, MD 20892, USA.
Xinyu WenOncogenomics Section, Genetics Branch, CCR, NCI, NIH, Bethesda, MD 20892, USA.
Swetha ParvathaneniDepartment of Biochemistry and Molecular Biology, College of Medicine, Howard University, Washington, DC 20059, USA.
Dickran KazandjianMolecular Genetics and Carcinogenesis Section, Laboratory of Human Carcinogenesis, CCR, NCI, NIH, Bethesda, MD 20892, USA.
Lisa M JenkinsLaboratory of Cell Biology, CCR, NCI, NIH, Bethesda, MD 20892, USA.
Wei TangMolecular Epidemiology Section, Laboratory of Human Carcinogenesis, CCR, NCI, NIH, Bethesda, MD 20892, USA.
Fathi ElloumiOffice of Science and Technology Resources, CCR, NCI, NIH, Bethesda, MD 20892, USA.
Jennifer L MartindaleLaboratory of Genetics, National Institute on Aging-Intramural Research Program, NIH, Baltimore, MD 21224, USA.
Maite HuarteCenter for Applied Medical Research, Department of Gene Therapy and Regulation of Gene Expression, University of Navarra, 31008 Pamplona, Spain.
Yuelin ZhuMolecular Genetics Section, Genetics Branch, CCR, NCI, NIH, Bethesda, MD 28092, USA.
Ana I RoblesMolecular Genetics and Carcinogenesis Section, Laboratory of Human Carcinogenesis, CCR, NCI, NIH, Bethesda, MD 20892, USA.
Susan M FrierIonis Pharmaceuticals, Carlsbad, CA 92010, USA.
Frank RigoIonis Pharmaceuticals, Carlsbad, CA 92010, USA.
Maggie CamOffice of Science and Technology Resources, CCR, NCI, NIH, Bethesda, MD 20892, USA.
Stefan AmbsMolecular Epidemiology Section, Laboratory of Human Carcinogenesis, CCR, NCI, NIH, Bethesda, MD 20892, USA.
Sudha SharmaDepartment of Biochemistry and Molecular Biology, College of Medicine, Howard University, Washington, DC 20059, USA.
Curtis C HarrisMolecular Genetics and Carcinogenesis Section, Laboratory of Human Carcinogenesis, CCR, NCI, NIH, Bethesda, MD 20892, USA.
Mary DassoLaboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA.
Kannanganattu V PrasanthDepartment of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Ashish LalRegulatory RNAs and Cancer Section, Genetics Branch, Center for Cancer Research (CCR), National Cancer Institute (NCI), NIH, Bethesda, MD 20892, USA. Electronic address: ashish.lal@nih.gov.
Center for Cancer Research · USNational Cancer Institute · USUniversity of Illinois Urbana-Champaign · USEunice Kennedy Shriver National Institute of Child Health and Human Development · USGenesis Laboratories · USHoward University · USIonis Pharmaceuticals (United States) · USOffice of Science · USNational Institute on Aging · USUniversidad de Navarra · ES

Funding

Mitotic roles of the Nuclear Transport MachineryZIAHD008954 · NICHD · EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT · PI DASSO, MARY C. · 2015 to 2025
$25.3M
Precision Medicine of CancerZIABC011492 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI HARRIS, CURTIS · 2013 to 2025
$23.2M
Role of long non-coding RNAs in p53 signalingZIABC011646 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI LAL, ASHISH · 2015 to 2025
$15.0M
PROTEOMICS CORE FACILITYG12MD007597 · NIMHD · HOWARD UNIVERSITY · PI SOUTHERLAND, WILLIAM M. · 2012 to 2018
$14.8M
Molecular Analysis of RECQ1 Functions in Genome MaintenanceSC1GM093999 · NIGMS · HOWARD UNIVERSITY · PI SHARMA, SUDHA · 2010 to 2019
$3.0M
Characterization of nuclear retained RNA-mediated gene regulatory mechanismR01GM088252 · NIGMS · UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN · PI KANNANGANATTU, PRASANTH KUMAR VIJAYAN · 2011 to 2015
$1.4M
Intramural NIH HHS ZIA BC011646NIGMS NIH HHS R01 GM088252NIGMS NIH HHS SC1 GM093999NIMHD NIH HHS G12 MD007597
6 · The paper itself

Abstract

Basal p53 levels are tightly suppressed under normal conditions. Disrupting this regulation results in elevated p53 levels to induce cell cycle arrest, apoptosis, and tumor suppression. Here, we report the suppression of basal p53 levels by a nuclear, p53-regulated long noncoding RNA that we termed PURPL (p53 upregulated regulator of p53 levels). Targeted depletion of PURPL in colorectal cancer cells results in elevated basal p53 levels and induces growth defects in cell culture and in mouse xenografts. PURPL associates with MYBBP1A, a protein that binds to and stabilizes p53, and inhibits the formation of the p53-MYBBP1A complex. In the absence of PURPL, MYBBP1A interacts with and stabilizes p53. Silencing MYBBP1A significantly rescues basal p53 levels and proliferation in PURPL-deficient cells, suggesting that MYBBP1A mediates the effect of PURPL in regulating p53. These results reveal a p53-PURPL auto-regulatory feedback loop and demonstrate a role for PURPL in maintaining basal p53 levels.

Indexed as

ApoptosisCell CycleCell Cycle CheckpointsCell LineCell Line, TumorCell ProliferationColorectal NeoplasmsDNA-Binding ProteinsELAV-Like Protein 1HCT116 CellsHumansNuclear ProteinsNucleocytoplasmic Transport ProteinsRNA-Binding ProteinsRNA, Long NoncodingTranscription FactorsDNA-Binding ProteinsELAV-Like Protein 1MYBBP1A protein, humanNuclear ProteinsNucleocytoplasmic Transport ProteinsRNA-Binding ProteinsRNA, Long NoncodingTP53 protein, humanTranscription FactorsTumor Suppressor Protein p53CRCHuRLINC01021lincRNAlncRNALOC643401MYBBP1Ap53PURPLRP11-46C20.1

Identifiers

PMID28877474
PMCPMC5777516
OpenAlexW2752927240

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.