ArticleThe Journal of cell biology1987
Acidification of the cytosol inhibits endocytosis from coated pits.
Article in The Journal of cell biology, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 118 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
118 citing papers in PubMed, 342 citations in OpenAlex.
- Functional Specialization of Clathrin-Independent Endocytic Routes: Mechanisms and Cellular Roles.Traffic (Copenhagen, Denmark) · 2026Review
- LDHB Overexpression Can Partially Overcome T Cell Inhibition by Lactic Acid.International journal of molecular sciences · 2022Article
- Review
- Understanding nanoparticle endocytosis to improve targeting strategies in nanomedicine.Chemical Society reviews · 2021Review
- Diacylglycerol kinase and phospholipase D inhibitors alter the cellular lipidome and endosomal sorting towards the Golgi apparatus.Cellular and molecular life sciences : CMLS · 2021Article
- Article
- Measuring Endocytosis During Proliferative Cell Quiescence.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Dynamic measurement of cytosolic pH and [NOProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Biological response and cytotoxicity induced by lipid nanocapsules.Journal of nanobiotechnology · 2020Article
- Genome-Wide Identification of Genes Involved in General Acid Stress and Fluoride Toxicity inFrontiers in microbiology · 2020Article
- PH-dependent cell-cell interactions in the green alga Chara.Protoplasma · 2019Article
- Spoiled for Choice: Diverse Endocytic Pathways Function at the Cell Surface.Annual review of cell and developmental biology · 2019Review
- Clathrin-independent endocytosis: an increasing degree of complexity.Histochemistry and cell biology · 2018Review
- Multifactorial Regulation of G Protein-Coupled Receptor Endocytosis.Biomolecules & therapeutics · 2017Review
- Acidic pH Is a Metabolic Switch for 2-Hydroxyglutarate Generation and Signaling.The Journal of biological chemistry · 2016Article
- Mitochondrial uncouplers inhibit clathrin-mediated endocytosis largely through cytoplasmic acidification.Nature communications · 2016Article
- Building endocytic pits without clathrin.Nature reviews. Molecular cell biology · 2015Review
- Increases in intracellular pH facilitate endocytosis and decrease availability of voltage-gated proton channels in osteoclasts and microglia.The Journal of physiology · 2013Article
- Retrograde transport of protein toxins through the Golgi apparatus.Histochemistry and cell biology · 2013Review
- Uptake of ricinB-quantum dot nanoparticles by a macropinocytosis-like mechanism.Journal of nanobiotechnology · 2012Article
58 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Acidification of the cytosol of a number of different cell lines strongly reduced the endocytic uptake of transferrin and epidermal growth factor. The number of transferrin binding sites at the cell surface was increased in acidified cells. Electron microscopic studies showed that the number of coated pits at the cell surface was not reduced in cells with acidified cytosol. Experiments with transferrin-horseradish peroxidase conjugates and a monoclonal anti-transferrin receptor antibody demonstrated that transferrin receptors were present in approximately 75% of the coated pits both in control cells and in cells with acidified cytosol. The data therefore indicate that the reason for the reduced endocytic uptake of transferrin at internal pH less than 6.5 is an inhibition of the pinching off of coated vesicles. In contrast, acidification of the cytosol had only little effect on the uptake of ricin and the fluid phase marker lucifer yellow. Ricin endocytosed by cells with acidified cytosol exhibited full toxic effect on the cells. Although the pathway of this uptake in acidified cells remains uncertain, some coated pits may still be involved. However, the data are also consistent with the possibility that an alternative endocytic pathway involving smooth (uncoated) pits exists.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.