ArticleGenesis (New York, N.Y. : 2000)2017
A new mouse line for cell ablation by diphtheria toxin subunit A controlled by a Cre-dependent FLEx switch.
Article in Genesis (New York, N.Y. : 2000), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed.
- Assessing leaky expression of Cre-dependent DNA constructs in the mouse genome using sensitive bioluminescent reporters.Cell reports methods · 2026Article
- Article
- Altered lipid metabolism promoting cardiac fibrosis is mediated by CD34Experimental & molecular medicine · 2024Article
- Stem cell antigen-1Cellular and molecular life sciences : CMLS · 2023Article
- High-efficiency pharmacogenetic ablation of oligodendrocyte progenitor cells in the adult mouse CNS.Cell reports methods · 2023Article
- Single cell and lineage tracing studies reveal the impact of CD34Stem cell research & therapy · 2023Article
- A direct excitatory projection from entorhinal layer 6b neurons to the hippocampus contributes to spatial coding and memory.Nature communications · 2022Article
- Proneural genes define ground-state rules to regulate neurogenic patterning and cortical folding.Neuron · 2021Article
- Versatile cell ablation tools and their applications to study loss of cell functions.Cellular and molecular life sciences : CMLS · 2019Review
- Macrophages restrict the nephrogenic field and promote endothelial connections during kidney development.eLife · 2019Article
- Neural Circuitry Underlying Waking Up to Hypercapnia.Frontiers in neuroscience · 2019Review
- Overview of the reporter genes and reporter mouse models.Animal models and experimental medicine · 2018Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Recombinase responsive mouse lines expressing diphtheria toxin subunit A (DTA) are well established tools for targeted ablation of genetically defined cell populations. Here we describe a new knock-in allele at the Gt(Rosa)26Sor locus that retains the best features of previously described DTA alleles-including a CAG promoter, attenuated mutant DTA cDNA, and ubiquitous EGFP labeling-with the addition of a Cre-dependent FLEx switch for tight control of expression. The FLEx switch consists of two pairs of antiparallel lox sites requiring Cre-mediated recombination for inversion of the DTA to the proper orientation for transcription. We demonstrate its utility by Cre-dependent ablation of both a broad domain in the embryonic nervous system and a discrete population of cells in the fetal gonads. We conclude that this new DTA line is useful for targeted ablation of genetically-defined cell populations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.