Evidence map›Paper›PMID 28874184›Full record

ArticleStem cell research & therapy2017

Multipotent stromal cells/mesenchymal stem cells and fibroblasts combine to minimize skin hypertrophic scarring.

Cecelia C Yates, Melanie Rodrigues, Austin Nuschke, Zariel I Johnson, Diana Whaley, Donna Stolz, Joseph Newsome, Alan Wells

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research & therapy, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
33citing papers in PubMed, 1 pooled it
6.9field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

33 citing papers in PubMed, 1 synthesis or guideline pooled it, 68 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Cecelia C YatesDepartment of Pathology, University of Pittsburgh, 3550 Terrace St., Scaife Hall, S-713, Pittsburgh, PA, 15261, USA. cey4@pitt.edu.
Melanie RodriguesDepartment of Plastic Surgery, Stanford University, Stanford, CA, USA.
Austin NuschkeDepartment of Pathology, University of Pittsburgh, 3550 Terrace St., Scaife Hall, S-713, Pittsburgh, PA, 15261, USA.
Zariel I JohnsonDepartment of Pathology, University of Pittsburgh, 3550 Terrace St., Scaife Hall, S-713, Pittsburgh, PA, 15261, USA.
Diana WhaleyPittsburgh VAMC, Pittsburgh, PA, USA.
Donna StolzDepartment of Cell Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Joseph NewsomeDepartment of Pathology, University of Pittsburgh, 3550 Terrace St., Scaife Hall, S-713, Pittsburgh, PA, 15261, USA.
Alan WellsDepartment of Pathology, University of Pittsburgh, 3550 Terrace St., Scaife Hall, S-713, Pittsburgh, PA, 15261, USA. wellsa@upmc.edu.
University of Pittsburgh · USVA Pittsburgh Healthcare System · USStanford University · US

Funding

Spatial Segregation of Cell Functioning During MotilityR01GM069668 · NIGMS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI WELLS, ALAN · 2004 to 2019
$4.7M
Dermal-Epidermal Communication during Wound HealingR01GM063569 · NIGMS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI WELLS, ALAN · 2003 to 2018
$3.7M
Dermal-Epidermal Communication during Wound HealingR56GM063569 · NIGMS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI WELLS, ALAN · 2008 to 2008
$341k
NIGMS NIH HHS R01 GM063569NIGMS NIH HHS R01 GM069668NIGMS NIH HHS R56 GM063569
6 · The paper itself

Abstract

backgroundTransplantation of mesenchymal stem cells (MSC) has been proposed to improve wound healing. However, as these cells only transiently survive in the implantation site, the mechanisms underlying this beneficial healing response are associated with restorative paracrine effects of MSC matricellular factors on resident stromal cells. However, this requires that the recipient has a robust reservoir of viable cells. Here, we examine the influence of MSCs on the behavior of cotransplanted fibroblasts, in a manner to provide augmented cellular reserve to debilitated individuals, specifically focusing on matrix remodeling following in-vivo wounding.

methodsUsing a Hylan-A dermal filler hydrogel containing collagen I and tenascin-C for delivery and increased survival of transplanted cells, we find that cotransplantation of MSCs with fibroblasts reduces scarring.

resultsTransplanted xenogeneic MSCs augmented fibroblast proliferation, migration, and extracellular matrix deposition critical for wound closure, and reduced inflammation following wounding. MSCs also corrected matrix remodeling by CXCR3-deficient fibroblasts which otherwise led to hypertrophic scarring. This effect was superior to MSC or fibroblast transplantation alone.

conclusionsTaken together, these data suggest that MSCs, even if eventually rejected, transplanted with fibroblasts normalize matrix regeneration during healing. The current study provides insight into cellular therapies as a viable method for antifibrotic treatment and demonstrates that even transiently engrafted cells can have a long-term impact via matrix modulation and education of other tissue cells.

Indexed as

Mesenchymal Stem Cell TransplantationWound HealingAnimalsCell- and Tissue-Based TherapyCell CommunicationCelluloseCicatrix, HypertrophicCoculture TechniquesDrug CombinationsExtracellular MatrixFemaleFibroblastsGene DeletionGene ExpressionHexamethonium CompoundsHyaluronic AcidCelluloseCxcr3 protein, mouseDrug CombinationsHexamethonium CompoundsHyaluronic Acidhylan gelReceptors, CXCR3TantalumThrombinCell therapyCollagen and epidermal–dermal communicationExtracellular matrixFibroblastMesenchymal stromal cellsTenascin-C

Identifiers

PMID28874184
PMCPMC5585998
OpenAlexW2751632681

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.