ArticlePloS one2017
DNA in a bottle-Rapid metabarcoding survey for early alerts of invasive species in ports.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Pooled it
- Meta-analysis shows both congruence and complementarity of DNA and eDNA metabarcoding to traditional methods for biological community assessment.Molecular ecology · 2022Pooled it
- Article
- Are Sponges Good Natural Sentinels for Monitoring Fish Diversity in Antarctic Coastal Waters?Ecology and evolution · 2025Article
- Microbiome of wild oysters: assessing diversity and detecting potential pathogens using a metabarcoding approach.BMC microbiology · 2025Article
- Environmental DNA and Hydroacoustic Surveys for Monitoring the Spread of the Invasive European Catfish (Animals : an open access journal from MDPI · 2025Article
- No pump, no problem: evaluating passive eDNA sampling for marine biomonitoring of a nuisance macroalga.PeerJ · 2025Article
- Upscaling biodiversity monitoring: Metabarcoding estimates 31,846 insect species from Malaise traps across Germany.Molecular ecology resources · 2025Article
- A new sampling device for metabarcoding surveillance of port communities and detection of non-indigenous species.iScience · 2024Article
- Article
- Harnessing environmental DNA to reveal biogeographical patterns of non-indigenous species for improved co-governance of the marine environment in Aotearoa New Zealand.Scientific reports · 2023Article
- Ecosystems monitoring powered by environmental genomics: A review of current strategies with an implementation roadmap.Molecular ecology · 2021Review
- eDNA metabarcoding of small plankton samples to detect fish larvae and their preys from Atlantic and Pacific waters.Scientific reports · 2021Article
- A novel metabarcoding primer pair for environmental DNA analysis of Cephalopoda (Mollusca) targeting the nuclear 18S rRNA region.Royal Society open science · 2021Article
- Assessing the efficacy of eDNA metabarcoding for measuring microbial biodiversity within forest ecosystems.Scientific reports · 2021Article
- Article
- High-Throughput Sequencing of Environmental DNA as a Tool for Monitoring Eukaryotic Communities and Potential Pathogens in a Coastal Upwelling Ecosystem.Frontiers in veterinary science · 2021Article
- Targeted and passive environmental DNA approaches outperform established methods for detection of quagga mussels,Ecology and evolution · 2020Article
- Monitoring of biofouling communities in a Portuguese port using a combined morphological and metabarcoding approach.Scientific reports · 2020Article
- Assessing insect biodiversity with automatic light traps in Brazil: Pearls and pitfalls of metabarcoding samples in preservative ethanol.Ecology and evolution · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biota monitoring in ports is increasingly needed for biosecurity reasons and safeguarding marine biodiversity from biological invasion. Present and future international biosecurity directives can be accomplished only if the biota acquired by maritime traffic in ports is controlled. Methodologies for biota inventory are diverse and now rely principally on extensive and labor-intensive sampling along with taxonomic identification by experts. In this study, we employed an extremely simplified environmental DNA (eDNA) sampling methodology from only three 1-L bottles of water per port, followed by metabarcoding (high-throughput sequencing and DNA-based species identification) using 18S rDNA and Cytochrome oxidase I as genetic barcodes. Eight Bay of Biscay ports with available inventory of fouling invertebrates were employed as a case study. Despite minimal sampling efforts, three invasive invertebrates were detected: the barnacle Austrominius modestus, the tubeworm Ficopomatus enigmaticus and the polychaete Polydora triglanda. The same species have been previously found from visual and DNA barcoding (genetic identification of individuals) surveys in the same ports. The current costs of visual surveys, conventional DNA barcoding and this simplified metabarcoding protocol were compared. The results encourage the use of metabarcoding for early biosecurity alerts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.