Evidence map›Paper›PMID 28873262›Full record

ArticlePediatric blood & cancer2018

Reactivation of TWIST1 contributes to Ewing sarcoma metastasis.

Sun Choo, Ping Wang, Robert Newbury, William Roberts, Jing Yang

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Pediatric blood & cancer, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
1.8field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 21 citations in OpenAlex.

  1. Review
  2. Article
  3. Opposite patterns of association of TWIST1 expression with patient survival in SHH and Group 4 medulloblastoma.Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery · 2025
    Article
  4. Article
  5. Article
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  7. Review
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  9. Review
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  12. [Twist regulates proliferation, migration and invasion of osteosarcoma cells in vitro].Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2018
    Article
  13. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Sun ChooDivision of Hematology/Oncology, Department of Pediatrics, University of California, San Diego, California.ORCID http://orcid.org/0000-0001-8529-4447
Ping WangDepartment of Pharmacology, University of California San Diego, San Diego, California.
Robert NewburyDepartment of Pathology, University of California San Diego, San Diego, California.
William RobertsDivision of Hematology/Oncology, Department of Pediatrics, University of California, San Diego, California.
Jing YangDivision of Hematology/Oncology, Department of Pediatrics, University of California, San Diego, California.
University of San Diego · USUniversity of California San Diego · US

Funding

Regulation of Tumor Invasion and Metastasis by Matrix StiffnessR01CA174869 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Jing Yang · 2015 to 2026
$4.3M
Regulation of Tumor Invasion by Twist1R01CA168689 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI YANG, JING · 2012 to 2016
$1.7M
NCI NIH HHS R01 CA168689NCI NIH HHS R01 CA174869
6 · The paper itself

Abstract

backgroundEwing sarcoma is a cancer of bone and soft tissue. Despite aggressive treatment, survival remains poor, particularly in patients with metastatic disease. Failure to treat Ewing sarcoma is due to the lack of understanding of the molecular pathways that regulate metastasis. In addition, no molecular prognostic markers have been identified for Ewing sarcoma to risk stratify patients. PROCEDURE: Ewing sarcoma patients were divided into high or low Twist1 gene expression and survival curves were generated using the R2 microarray-based Genomic Analysis platform (http://r2.amc.nl). Tumors from Ewing sarcoma patients were also evaluated for TWIST1 expression by immunohistochemistry. Ewing sarcoma xenografts were established to evaluate the role of TWIST1 in metastasis. The effects of Twist1 on migration and invasion were evaluated using migration and invasion assays in A673 and RDES cells.

resultsTwist1 expression was a negative prognostic marker for overall survival in a public Ewing sarcoma patient data set based on Twist1 mRNA levels and in patient tumor samples based on Twist1 immunohistochemistry. TWIST1 is detected in significantly higher percentage of patients with metastatic diseases than localized disease. Using Ewing sarcoma tumor xenografts in mice, we found that suppressing TWIST1 levels suppressed metastasis without affecting primary tumor development. Knockdown of Twist1 inhibited the migration and invasion capability, while overexpression of Twist1 promoted migration and invasion in Ewing sarcoma cells.

conclusionThese results suggest that TWIST1 promotes metastasis in Ewing sarcoma and could be used as a prognostic marker for treatment stratification; however, further validation is required in a larger cohort of patients.

Indexed as

Cell MovementGene Expression Regulation, NeoplasticAnimalsBone NeoplasmsCell Line, TumorFemaleHeterograftsHumansMaleMiceNeoplasm InvasivenessNeoplasm MetastasisNeoplasm ProteinsNeoplasm TransplantationNuclear ProteinsSarcoma, EwingNeoplasm ProteinsNuclear ProteinsTWIST1 protein, humanTwist-Related Protein 1Ewing sarcomametastasisTWIST1

Identifiers

PMID28873262
PMCPMC5759052
OpenAlexW2750663163

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.