ArticleJournal of visualized experiments : JoVE2017
Characterizing Cell Migration Within Three-dimensional In Vitro Wound Environments.
Article in Journal of visualized experiments : JoVE, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed.
- Biological Models for Evaluating Hydrogel-Based Formulations in Wound Healing.Gels (Basel, Switzerland) · 2025Review
- Characterisation and correction of polarisation effects in fluorescently labelled fibres.Journal of microscopy · 2025Article
- Towards a more objective and high-throughput spheroid invasion assay quantification method.Scientific reports · 2024Article
- Towards a More Objective and High-throughput Spheroid Invasion Assay Quantification Method.bioRxiv : the preprint server for biology · 2024Article
- The Role of Fibroblasts in Skin Homeostasis and Repair.Biomedicines · 2024Review
- Microporous Annealed Particle (MAP) Scaffold Pore Size Influences Mesenchymal Stem Cell Metabolism and Proliferation Without Changing CD73, CD90, and CD105 Expression Over Two Weeks.Advanced biology · 2024Article
- In silico optimization of heparin microislands in microporous annealed particle hydrogel for endothelial cell migration.Acta biomaterialia · 2022Article
- Longitudinal shear wave elasticity measurements of millimeter-sized biomaterials using a single-element transducer platform.PloS one · 2022Article
- Spatially heterogeneous epidermal growth factor release from microporous annealed particle (MAP) hydrogel for improved wound closure.Journal of materials chemistry. B · 2021Article
- Heparin Microislands in Microporous Annealed Particle Scaffolds for Accelerated Diabetic Wound Healing.Advanced functional materials · 2021Article
- Modeling and Parameter Subset Selection for Fibrin Polymerization Kinetics with Applications to Wound Healing.Bulletin of mathematical biology · 2021Article
- Microphysiological systems for the modeling of wound healing and evaluation of pro-healing therapies.Journal of materials chemistry. B · 2020Review
- Assessment of Migration of Human MSCs through Fibrin Hydrogels as a Tool for Formulation Optimisation.Materials (Basel, Switzerland) · 2018Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Currently, most in vitro models of wound healing, such as well-established scratch assays, involve studying cell migration and wound closure on two-dimensional surfaces. However, the physiological environment in which in vivo wound healing takes place is three-dimensional rather than two-dimensional. It is becoming increasingly clear that cell behavior differs greatly in two-dimensional vs. three-dimensional environments; therefore, there is a need for more physiologically relevant in vitro models for studying cell migration behaviors in wound closure. The method described herein allows for the study of cell migration in a three-dimensional model that better reflects physiological conditions than previously established two-dimensional scratch assays. The purpose of this model is to evaluate cell outgrowth via the examination of cell migration away from a spheroid body embedded within a fibrin matrix in the presence of pro- or anti-migratory factors. Using this method, cell outgrowth from the spheroid body in a three-dimensional matrix can be observed and is easily quantifiable over time via brightfield microscopy and analysis of spheroid body area. The effect of pro-migratory and/or inhibitory factors on cell migration can also be evaluated in this system. This method provides researchers with a simple method of analyzing cell migration in three-dimensional wound associated matrices in vitro, thus increasing the relevance of in vitro cell studies prior to the use of in vivo animal models.
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Registered trials
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