ArticleNature communications2017
Engineering a riboswitch-based genetic platform for the self-directed evolution of acid-tolerant phenotypes.
Article in Nature communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- RATEX: A Scalable RNA-Based Platform for Logical and Multi-Layered Cellular Programming.Angewandte Chemie (International ed. in English) · 2026Article
- Engineering ligands for theophylline riboswitches expands its regulatory dynamic range in prokaryotic and eukaryotic systems.Nature communications · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Regulation of Duplex Formation in Response to pH and Complementary RNA: Development of Oligonucleotides Modified withACS omega · 2026Article
- Toehold Switch-Based Approach for Engineering Acid-Tolerance Modules to Enhance Production Robustness of Industrial E. coli Strains at Low pH.Microbial biotechnology · 2025Article
- Optimization of Capillary Vibrating Sharp-Edge Spray Ionization for Native Mass Spectrometry of Triplex DNA.ACS omega · 2025Article
- Harnessing microbial heterogeneity for improved biosynthesis fueled by synthetic biology.Synthetic and systems biotechnology · 2025Review
- Advances in stress-tolerance elements for microbial cell factories.Synthetic and systems biotechnology · 2024Review
- Synthetic translational coupling element for multiplexed signal processing and cellular control.Nucleic acids research · 2024Article
- Metabolic engineering for single-cell protein production from renewable feedstocks and its applications.Advanced biotechnology · 2024Review
- Dual genetic level modification engineering accelerate genome evolution of Corynebacterium glutamicum.Nucleic acids research · 2024Article
- Customizing cellular signal processing by synthetic multi-level regulatory circuits.Nature communications · 2023Review
- An Intelligent Synthetic Bacterium for Chronological Toxicant Detection, Biodegradation, and Its Subsequent Suicide.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Review
- Computational Methods for the Design of Recombinase Logic Circuits with Adaptable Circuit Specifications.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Engineering Ag43 Signal Peptides with Bacterial Display and Selection.Methods and protocols · 2022Article
- Exploiting spatial dimensions to enable parallelized continuous directed evolution.Molecular systems biology · 2022Article
- Inducible directed evolution of complex phenotypes in bacteria.Nucleic acids research · 2022Article
- Engineering Escherichia coli biofilm to increase contact surface for shikimate and L-malate production.Bioresources and bioprocessing · 2021Article
- The challenges and prospects of Escherichia coli as an organic acid production host under acid stress.Applied microbiology and biotechnology · 2021Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Environmental pH is a fundamental signal continuously directing the metabolism and behavior of living cells. Programming the precise cellular response toward environmental pH is, therefore, crucial for engineering cells for increasingly sophisticated functions. Herein, we engineer a set of riboswitch-based pH-sensing genetic devices to enable the control of gene expression according to differential environmental pH. We next develop a digital pH-sensing system to utilize the analogue-sensing behavior of these devices for high-resolution recording of host cell exposure to discrete external pH levels. The application of this digital pH-sensing system is demonstrated in a genetic program that autonomously regulated the evolutionary engineering of host cells for improved tolerance to a broad spectrum of organic acids, a valuable phenotype for metabolic engineering and bioremediation applications.Cells are exposed to shifts in environmental pH, which direct their metabolism and behavior. Here the authors design pH-sensing riboswitches to create a gene expression program, digitalize the system to respond to a narrow pH range and apply it to evolve host cells with improved tolerance to a variety of organic acids.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.