ArticleDevelopment (Cambridge, England)2017
A stepwise model of reaction-diffusion and positional information governs self-organized human peri-gastrulation-like patterning.
Article in Development (Cambridge, England), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 84 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
84 citing papers in PubMed, 1 synthesis or guideline pooled it, 170 citations in OpenAlex.
- Current strategies and opportunities to manufacture cells for modeling human lungs.Advanced drug delivery reviews · 2020Pooled it
- Acoustic cell patterning reveals geometry- and substrate-dependent vasculogenesis and human embryo model development.Nature communications · 2026Article
- The embryo game uncovers hidden cell behaviours.Nature communications · 2026Article
- Interpretable decoding of cell fate from a snapshot of combinatorial signaling.bioRxiv : the preprint server for biology · 2026Article
- Size-Modulated Mesoderm-Endoderm Divergence and Myocardial Cavitation in Micropatterned Cardioids.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Self-organized anteroposterior regionalization of early midbrain and hindbrain/spinal cords using micropatterned human pluripotent stem cells.SLAS technology · 2026Article
- From genes to germ layers: virtual twins of gastruloids.NPJ systems biology and applications · 2026Review
- Culture of pluripotent stem cells in microscale droplets modulates differentiation and tissue patterning towards organoids on chip.Stem cell research & therapy · 2025Article
- Advances in engineered models of peri-gastrulation.iScience · 2025Review
- The building blocks of embryo models: embryonic and extraembryonic stem cells.Cell discovery · 2025Review
- Templated Pluripotent Stem Cell Differentiation via Substratum-Guided Artificial Signaling.ACS biomaterials science & engineering · 2024Article
- Widespread biochemical reaction networks enable Turing patterns without imposed feedback.Nature communications · 2024Article
- Advances in micropatterning technology for mechanotransduction research.Mechanobiology in medicine · 2024Review
- Article
- ETV4 is a mechanical transducer linking cell crowding dynamics to lineage specification.Nature cell biology · 2024Article
- ARTseq-FISH reveals position-dependent differences in gene expression of micropatterned mESCs.Nature communications · 2024Article
- Time-integrated BMP signaling determines fate in a stem cell model for early human development.Nature communications · 2024Article
- Morphogen-driven differentiation is precluded by physical confinement in human iPSCs spheroids.Frontiers in bioengineering and biotechnology · 2024Article
- Recent advances in lung organoid development and applications in disease modeling.The Journal of clinical investigation · 2023Review
- Loss of TJP1 disrupts gastrulation patterning and increases differentiation toward the germ cell lineage in human pluripotent stem cells.Developmental cell · 2023Article
24 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
How position-dependent cell fate acquisition occurs during embryogenesis is a central question in developmental biology. To study this process, we developed a defined, high-throughput assay to induce peri-gastrulation-associated patterning in geometrically confined human pluripotent stem cell (hPSC) colonies. We observed that, upon BMP4 treatment, phosphorylated SMAD1 (pSMAD1) activity in the colonies organized into a radial gradient. We developed a reaction-diffusion (RD)-based computational model and observed that the self-organization of pSMAD1 signaling was consistent with the RD principle. Consequent fate acquisition occurred as a function of both pSMAD1 signaling strength and duration of induction, consistent with the positional-information (PI) paradigm. We propose that the self-organized peri-gastrulation-like fate patterning in BMP4-treated geometrically confined hPSC colonies arises via a stepwise model of RD followed by PI. This two-step model predicted experimental responses to perturbations of key parameters such as colony size and BMP4 dose. Furthermore, it also predicted experimental conditions that resulted in RD-like periodic patterning in large hPSC colonies, and rescued peri-gastrulation-like patterning in colony sizes previously thought to be reticent to this behavior.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.