ArticleUltramicroscopy2018
Estimating the effect of finite depth of field in single-particle cryo-EM.
Article in Ultramicroscopy, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed.
- Article
- Bridging the Structural Gap: A Methodological Review of Cryo-Electron Microscopy for Underrepresented Viruses.Viruses · 2026Review
- The relationship between the Ewald sphere and exit wave explored using focal series electron micrographs.IUCrJ · 2026Article
- Validation of 3D cryoEM single-particle reconstruction correctness and handedness with Ewald's sphere correction.Structural dynamics (Melville, N.Y.) · 2025Article
- Validation of 3D cryoEM single particle reconstruction correctness and handedness with Ewald's sphere correction.bioRxiv : the preprint server for biology · 2025Article
- Electron Tomography as a Tool to Study SARS-CoV-2 Morphology.International journal of molecular sciences · 2024Review
- Conventional Electron Microscopy, Cryogenic Electron Microscopy, and Cryogenic Electron Tomography of Viruses.Sub-cellular biochemistry · 2024Review
- The Ewald sphere/focus gradient does not limit the resolution of cryoEM reconstructions.Journal of structural biology: X · 2023Article
- Emerging Themes in CryoEM─Single Particle Analysis Image Processing.Chemical reviews · 2022Review
- Sub-2 Å Ewald curvature corrected structure of an AAV2 capsid variant.Nature communications · 2018Article
- Pushing the resolution limit by correcting the Ewald sphere effect in single-particle Cryo-EM reconstructions.Nature communications · 2018Article
- Ewald sphere correction using a single side-band image processing algorithm.Ultramicroscopy · 2018Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
The extent to which the resolution varies within a three-dimensional (3-D) reconstruction, when the diameter of an object is large, is investigated computationally. Numerical simulation is used to model ideal three-dimensional point-spread functions at different radial positions within an object. It is shown that reconstructed density maps are affected less than might have been expected when particles are larger than the depth of field. This favorable outcome is attributed mainly to the fact that a point which lies outside the depth of field relative to the center, for some orientations of the object, will also lie within the depth of field for other orientations. We find, as a result, that the diameter of a particle can be as much as four times the depth of field (as defined by a 90° phase-error criterion) before curvature of the Ewald sphere becomes a limiting factor in determining the resolution that can be achieved.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.