ArticleNature chemical biology2017
An artificial transport metabolon facilitates improved substrate utilization in yeast.
Article in Nature chemical biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 92 citations in OpenAlex.
- Engineering peroxisomal cofactor systems in Saccharomyces cerevisiae for enhanced biosynthesis of α-humulene.World journal of microbiology & biotechnology · 2026Article
- Systems-Level Metabolic Regulation Strategies of Xylose Metabolism in Saccharomyces cerevisiae.Biotechnology journal · 2026Review
- Genetically encoded biosensor enabled mining, characterisation and engineering of aromatic acid MFS transporters.Journal of biological engineering · 2025Article
- The biosynthesis and diversity of taxanes: From pathway elucidation to engineering and synthetic biology.Plant communications · 2025Review
- Big data mining, rational modification, and ancestral sequence reconstruction inferred multiple xylose isomerases for biorefinery.Science advances · 2023Article
- Screening ofFrontiers in microbiology · 2023Article
- Microbial synthesis of long-chain α-alkenes from methanol by engineering Pichia pastoris.Bioresources and bioprocessing · 2022Article
- Enzyme assemblies on demand.Nature chemical biology · 2022Article
- 2022 The 1st Western China symposium on the international frontier of synthetic biomanufacturing.Biotechnology notes (Amsterdam, Netherlands) · 2022Article
- Transporter Engineering in Microbial Cell Factory Boosts Biomanufacturing Capacity.Biodesign research · 2022Review
- Genetic fusion of P450 BM3 and formate dehydrogenase towards self-sufficient biocatalysts with enhanced activity.Scientific reports · 2021Article
- Blockade of fructose transporter protein GLUT5 inhibits proliferation of colon cancer cells: proof of concept for a new class of anti-tumor therapeutics.Pharmacological reports : PR · 2021Article
- An integrative approach to improving the biocatalytic reactions of whole cells expressing recombinant enzymes.World journal of microbiology & biotechnology · 2021Review
- Microtubule-assisted mechanism for toxisome assembly in Fusarium graminearum.Molecular plant pathology · 2021Article
- Successful Enzyme Colocalization Strategies in Yeast for Increased Synthesis of Non-native Products.Frontiers in bioengineering and biotechnology · 2021Review
- Transporter engineering in microbial cell factories: the ins, the outs, and the in-betweens.Current opinion in biotechnology · 2020Review
- Yeast as a promising heterologous host for steroid bioproduction.Journal of industrial microbiology & biotechnology · 2020Review
- Review
- Construction of advanced producers of first- and second-generation ethanol in Saccharomyces cerevisiae and selected species of non-conventional yeasts (Scheffersomyces stipitis, Ogataea polymorpha).Journal of industrial microbiology & biotechnology · 2020Review
- Designing minimalist membrane proteins.Biochemical Society transactions · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Efficient substrate utilization is the first and most important prerequisite for economically viable production of biofuels and chemicals by microbial cell factories. However, production rates and yields are often compromised by low transport rates of substrates across biological membranes and their diversion to competing pathways. This is especially true when common chassis organisms are engineered to utilize nonphysiological feedstocks. Here, we addressed this problem by constructing an artificial complex between an endogenous sugar transporter and a heterologous xylose isomerase in Saccharomyces cerevisiae. Direct feeding of the enzyme through the transporter resulted in acceleration of xylose consumption and substantially diminished production of xylitol as an undesired side product, with a concomitant increase in the production of ethanol. This underlying principle could also likely be implemented in other biotechnological applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.