ArticleeLife2017
A transgenic toolkit for visualizing and perturbing microtubules reveals unexpected functions in the epidermis.
Article in eLife, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 48 citations in OpenAlex.
- Mechanical constraints regulate embryonic stem cell mitosis in the developing brain.The EMBO journal · 2026Article
- Dynamic microtubules as sensors in animal cells.Biophysical journal · 2026Review
- Cell type-specific spatiotemporal control of GFP-tagged protein degradation in mice.The Journal of cell biology · 2026Article
- Technological advances in visualizing and rewiring microtubules during plant development.Journal of experimental botany · 2025Review
- Microtubule dependent changes in cell polarity are required for mammary epithelial cell migration and branching morphogenesis.Biology open · 2025Article
- A structured framework of cytoskeletal proteins and noncentrosomal microtubules promotes the initiation and elongation of invadopodia.Molecular biology of the cell · 2025Article
- Molecular and mechanical signatures contributing to mouse epidermal differentiation and barrier formation.eLife · 2025Article
- Control of epithelial tissue organization by mRNA localization.Nature communications · 2025Article
- Mechanisms of cortical microtubule organization in epidermal keratinocytes.Cellular and molecular life sciences : CMLS · 2025Article
- Cell cycle controls long-range calcium signaling in the regenerating epidermis.The Journal of cell biology · 2023Article
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- Desmosomal Cadherins in Health and Disease.Annual review of pathology · 2022Review
- Cytoskeletal control of early mammalian development.Nature reviews. Molecular cell biology · 2021Review
- Differentiated Daughter Cells Regulate Stem Cell Proliferation and Fate through Intra-tissue Tension.Cell stem cell · 2021Article
- A Niche Above: A Novel Modality of Stem Cell Regulation in Mammalian Skin Epidermis.Cell stem cell · 2021Article
- Roles for microtubules in the proliferative and differentiated cells of stratified epithelia.Current opinion in cell biology · 2021Review
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- Keratin 14-dependent disulfides regulate epidermal homeostasis and barrier function via 14-3-3σ and YAP1.eLife · 2020Article
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Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The physiological functions of microtubules (MTs) are poorly understood in many differentiated cell types. We developed a genetic toolkit to study MT dynamics and function in diverse cells. Using TRE-EB1-GFP mice, we found that MT dynamics are strongly suppressed in differentiated keratinocytes in two distinct steps due to alterations in both growth rate and lifetime. To understand the functions of these MT populations, we developed TRE-spastin mice to disrupt MTs in specific cell types. MT perturbation in post-mitotic keratinocytes had profound consequences on epidermal morphogenesis. We uncoupled cell-autonomous roles in cell flattening from non-cell-autonomous requirements for MTs in regulating proliferation, differentiation, and tissue architecture. This work uncovers physiological roles for MTs in epidermal development, and the tools described here will be broadly useful to study MT dynamics and functions in mammals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.