Integrated dielectrophoretic and surface plasmonic platform for million-fold improvement in the detection of fluorescent events.

Logeeshan Velmanickam, Michael Fondakowski, Ivan T Lima, Dharmakeerthi Nawarathna

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Article in Biomicrofluidics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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4 · The record

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5 · Who and what money

Authors and funding

4 authors.

Logeeshan VelmanickamDepartment of Electrical and Computer Engineering, North Dakota State University, Fargo, North Dakota 58102-6050, USA.

Michael FondakowskiDepartment of Mechanical Engineering, North Dakota State University, Fargo, North Dakota 58102-6050, USA.

Ivan T LimaDepartment of Electrical and Computer Engineering, North Dakota State University, Fargo, North Dakota 58102-6050, USA.ORCID 0000-0002-5184-3695

Dharmakeerthi NawarathnaDepartment of Electrical and Computer Engineering, North Dakota State University, Fargo, North Dakota 58102-6050, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We present an integrated dielectrophoretic (DEP) and surface plasmonic technique to quantify ∼1 pM of fluorescent molecules in low conductivity buffers. We have established a DEP force on target molecules to bring those molecules and place them on the nanometallic structures (hotspots) for quantification through surface plasmonic effects. Our results show that the DEP is capable of placing the fluorescent molecules on the hotspots, which are depicted as a significant reduction in the fluorescence lifetime of those molecules. To efficiently integrate the DEP and plasmonic effects, we have designed and utilized pearl-shaped interdigitated electrodes (PIDEs) in experiments. These electrodes generate 2-3 times higher DEP force than traditional interdigitated electrodes. Therefore, high-throughput assays can be developed. The nanometallic structures were strategically fabricated in the periphery of PIDEs for smooth integration of DEP and plasmonic detection. With the introduction of DEP, about 10

Identifiers

PMID28868108

DOI10.1063/1.5000008

PMCPMC5566558

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.