ArticleNeuroscience bulletin2018
An Efficient and Reliable Assay for Investigating the Effects of Hypoxia/Anoxia on Drosophila.
Article in Neuroscience bulletin, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Unravelling the Mechanism of N-Acetylcysteine in Alleviating Smoke-Induced Hypoxia-Driven Ocular Defects in Drosophila melanogaster.Molecular neurobiology · 2025Article
- Evaluation of Bioactive Effects of Five Plant Extracts with Different Phenolic Compositions against Different Therapeutic Targets.Antioxidants (Basel, Switzerland) · 2024Article
- Lifespan and ROS levels in different Drosophila melanogaster strains after 24 h hypoxia exposure.Biology open · 2022Article
- Increased Post-Hypoxic Oxidative Stress and Activation of the PERK Branch of the UPR inInternational journal of molecular sciences · 2021Article
- Axonemal Dynein DNAH5 is Required for Sound Sensation in Drosophila Larvae.Neuroscience bulletin · 2021Article
- Phosphorylation at S548 as a Functional Switch of Sterile Alpha and TIR Motif-Containing 1 in Cerebral Ischemia/Reperfusion Injury in Rats.Molecular neurobiology · 2021Article
- Pgant4 and Tango1 Mediate Anoxia and Reoxygenation Injury.Neuroscience bulletin · 2020Article
- Lysosomal Hydrolase Cathepsin D Non-proteolytically Modulates Dendritic Morphology in Drosophila.Neuroscience bulletin · 2020Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stroke is a leading cause of death worldwide. Up to one thousand potential drugs or interventions have been developed to treat stroke, out of which ~160 have gone on to clinical trials. However, none of them has been successful. New insights into the molecular and cellular mechanisms of ischemia-induced injury are needed for discovering new therapeutic targets. Recently, Drosophila has been used to uncover new hypoxia-related genes. In this study, we describe an efficient and reliable assay with a sophisticated apparatus for studying the effects of oxygen deprivation on flies. Using this assay, wild-type flies were exposed to an anoxic environment for varying lengths of time, then the cumulative death rate and mobility recovery were systematically analyzed. We found that anoxia for over one hour caused lethality. The cumulative death rate on day 5 after anoxia was linearly and positively correlated with the duration of anoxia, and reached 50% when the duration was 2.5 h-3 h. We also found that the mobility recovery in normoxia was slow, as the climbing ability remained largely unchanged 4 h-6 h after 2.5-h of anoxia. We suggest that 2.5 h-3 h of anoxia and 4 h-6 h of recovery before mobility analysis are appropriate for future use of the anoxia assay.
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