Evidence map›Paper›PMID 28866013›Full record

ArticleExperimental eye research2017

Contrasting cellular damage after Blue-IRIS and Femto-LASIK in cat cornea.

Kaitlin T Wozniak, Noah Elkins, Daniel R Brooks, Daniel E Savage, Scott MacRae, Jonathan D Ellis, Wayne H Knox, Krystel R Huxlin

Registry-linked trialAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental eye research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT03653715 (Clinical Performance Study of Clerio Single Vision and Bifocal Contact Lens Designs Immediately Following Insertion), which is not on this map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.7field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT03653715 nacompletednot on this mapstarted 2018, after this paper: background citation

Clinical Performance Study of Clerio Single Vision and Bifocal Contact Lens Designs Immediately Following Insertion

TypeinterventionalSponsorClerio Vision, Inc.Ran2018 to 2018Enrolled30ConditionsPresbyopiaArmsClerio Vision LIRIC-modified Bifocal Contact Lens, Johnson & Johnson 1-Day Acuvue Moist Multifocal Contact Lens, Johnson & Johnson 1-Day Acuvue Moist Contact Lens, Clerio Vision Single Vision Contact Lens
3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 11 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Kaitlin T WozniakThe Institute of Optics, University of Rochester, Rochester, NY 14627, USA.
Noah ElkinsDepartment of Biomedical Engineering, University of Rochester, Rochester, NY 14627, USA.
Daniel R BrooksThe Institute of Optics, University of Rochester, Rochester, NY 14627, USA.
Daniel E SavageThe Institute of Optics, University of Rochester, Rochester, NY 14627, USA; Center for Visual Science, University of Rochester, Rochester, NY 14627, USA; Flaum Eye Institute, University of Rochester, Rochester, NY 14627, USA.
Scott MacRaeCenter for Visual Science, University of Rochester, Rochester, NY 14627, USA; Flaum Eye Institute, University of Rochester, Rochester, NY 14627, USA.
Jonathan D EllisThe Institute of Optics, University of Rochester, Rochester, NY 14627, USA; Department of Mechanical Engineering, University of Rochester, Rochester, NY 14627, USA.
Wayne H KnoxThe Institute of Optics, University of Rochester, Rochester, NY 14627, USA; Center for Visual Science, University of Rochester, Rochester, NY 14627, USA.
Krystel R HuxlinCenter for Visual Science, University of Rochester, Rochester, NY 14627, USA; Flaum Eye Institute, University of Rochester, Rochester, NY 14627, USA. Electronic address: huxlin@cvs.rochester.edu.
University of Rochester · US

Funding

VISUAL SCIENCE RESEARCH CTR SUPPORTP30EY001319 · NEI · UNIVERSITY OF ROCHESTER · PI MICHELE RUCCI · 1985 to 2026
$17.5M
VISUAL SCIENCET32EY007125 · NEI · UNIVERSITY OF ROCHESTER · PI Krystel R Huxlin, Duje Tadin · 1990 to 2026
$7.1M
The role of PPAR{gamma} ligands in corneal wound healing and opticsR01EY015836 · NEI · UNIVERSITY OF ROCHESTER · PI HUXLIN, KRYSTEL R · 2004 to 2023
$7.1M
NEI NIH HHS P30 EY001319NEI NIH HHS R01 EY015836NEI NIH HHS T32 EY007125
6 · The paper itself

Abstract

Blue-intra-tissue refractive index shaping (Blue-IRIS) is a new approach to laser refractive correction of optical aberrations in the eye, which alters the refractive index of the cornea rather than changing its shape. Before it can be implemented in humans, it is critical to establish whether and to what extent, Blue-IRIS damages the cornea. Here, we contrasted the impact of -1.5 D cylinder refractive corrections inscribed using either Blue-IRIS or femtosecond laser in-situ keratomileusis (femto-LASIK) on corneal cell viability. Blue-IRIS was used to write a -1.5 D cylinder gradient index (GRIN) lens over a 2.5 mm by 2.5 mm area into the mid-stromal region of the cornea in six freshly-enucleated feline eyes. The same correction (-1.5 D cylinder) was inscribed into another four cat eyes using femto-LASIK. Six hours later, all corneas were processed for histology and stained for terminal deoxynucleotidyl transferase-mediated dUTP-digoxigenin nick end labeling (TUNEL) and p-γ-H2AX to label damaged cells. In Blue-IRIS-treated corneas, no tissue was removed and TUNEL-stained cells were confined to the laser focal zone in the stroma. In femto-LASIK, photoablation removed 14 μm of anterior stroma, but in addition, TUNEL-positive cells clustered across the femto-flap, the epithelium at the flap edges and the stroma below the ablation zone. Keratocytes positive for p-γ-H2AX were seen adjacent to all Blue-IRIS focal zones, but were completely absent from femto-LASIK-treated corneas. Unlike femto-LASIK, Blue-IRIS attains refractive correction in the cornea without tissue removal and only causes minimal, localized keratocyte death within the laser focal zones. In addition, Blue-IRIS induced DNA modifications associated with phosphorylation of γ-H2AX in keratocytes adjacent to the laser focal zones. We posit that this p-γ-H2AX response is related to alterations in chromatin structure caused by localized changes in osmolarity, a possible mechanism for the induced refractive index changes.

Indexed as

AnimalsCatsCell CountCorneaCorneal StromaDisease Models, AnimalIn Situ Nick-End LabelingKeratomileusis, Laser In SituLasers, ExcimerRefractive Surgical ProceduresGRIN lensPhotoablationRefractive correctionRefractive index modificationStromaTUNELγ-H2AX

Identifiers

PMID28866013
PMCPMC5705398
OpenAlexW2752670265

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Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.