Evidence map›Paper›PMID 28865476›Full record

ArticleJournal of neuroinflammation2017

Inhibition of hypothalamic leukemia inhibitory factor exacerbates diet-induced obesity phenotype.

Milena Fioravante, Bruna Bombassaro, Albina F Ramalho, Nathalia R Dragano, Joseane Morari, Carina Solon, Natalia Tobar, Celso D Ramos, Licio A Velloso

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of neuroinflammation, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.2field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 18 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Milena FioravanteLaboratory of Cell Signaling, University of Campinas, Campinas, São Paulo, 13084-970, Brazil.
Bruna BombassaroLaboratory of Cell Signaling, University of Campinas, Campinas, São Paulo, 13084-970, Brazil.
Albina F RamalhoLaboratory of Cell Signaling, University of Campinas, Campinas, São Paulo, 13084-970, Brazil.
Nathalia R DraganoLaboratory of Cell Signaling, University of Campinas, Campinas, São Paulo, 13084-970, Brazil.
Joseane MorariLaboratory of Cell Signaling, University of Campinas, Campinas, São Paulo, 13084-970, Brazil.
Carina SolonLaboratory of Cell Signaling, University of Campinas, Campinas, São Paulo, 13084-970, Brazil.
Natalia TobarDepartment of Radiology, University of Campinas, Campinas, São Paulo, 13084-970, Brazil.
Celso D RamosDepartment of Radiology, University of Campinas, Campinas, São Paulo, 13084-970, Brazil.
Licio A VellosoLaboratory of Cell Signaling, University of Campinas, Campinas, São Paulo, 13084-970, Brazil. lavelloso.unicamp@gmail.com.
Universidade Estadual de Campinas (UNICAMP) · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe consumption of large amounts of dietary fats can trigger an inflammatory response in the hypothalamus and contribute to the dysfunctional control of caloric intake and energy expenditure commonly present in obesity. The objective of this study was to identify chemokine-related transcripts that could be involved in the early stages of diet-induced hypothalamic inflammation.

methodsWe used immunoblot, PCR array, real-time PCR, immunofluorescence staining, glucose and insulin tolerance tests, and determination of general metabolic parameters to evaluate markers of inflammation, body mass variation, and glucose tolerance in mice fed a high-fat diet.

resultsUsing a real-time PCR array, we identified leukemia inhibitory factor as a chemokine/cytokine undergoing a rapid increase in the hypothalamus of obesity-resistant and a rapid decrease in the hypothalamus of obesity-prone mice fed a high-fat diet for 1 day. We hypothesized that the increased hypothalamic expression of leukemia inhibitory factor could contribute to the protective phenotype of obesity-resistant mice. To test this hypothesis, we immunoneutralized hypothalamic leukemia inhibitory factor and evaluated inflammatory and metabolic parameters. The immunoneutralization of leukemia inhibitory factor in the hypothalamus of obesity-resistant mice resulted in increased body mass gain and increased adiposity. Body mass gain was mostly due to increased caloric intake and reduced spontaneous physical activity. This modification in the phenotype was accompanied by increased expression of inflammatory cytokines in the hypothalamus. In addition, the inhibition of hypothalamic leukemia inhibitory factor was accompanied by glucose intolerance and insulin resistance.

conclusionHypothalamic expression of leukemia inhibitory factor may protect mice from the development of diet-induced obesity; the inhibition of this protein in the hypothalamus transforms obesity-resistant into obesity-prone mice.

Indexed as

PhenotypeAnimalsDiet, High-FatEnergy IntakeHypothalamusImmunoglobulin GLeukemia Inhibitory FactorMaleMiceObesityRandom AllocationImmunoglobulin GLeukemia Inhibitory FactorLif protein, mouseAdipose tissueDiabetesGlucoseInflammationInsulin

Identifiers

PMID28865476
PMCPMC5581454
OpenAlexW2751711793

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.