ArticlePloS one2017
Comprehensive analysis of amino acid sequence diversity at the F protein cleavage site of Newcastle disease virus in fusogenic activity.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
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Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it, 40 citations in OpenAlex.
- Insights into the multifunctionality of viral glycoproteins F and HN in the lifecycle and pathogenesis of Newcastle disease virus: a systematic review.Veterinary research · 2025Pooled it
- A genetically-distinct Pigeon Paramyxovirus-I isolate fails to cross-react with neutralizing antibodies raised by LaSota vaccine in chicken.Virus genes · 2026Article
- Evolution and Spread of Regionally Adapted Newcastle Disease Virus Isolates From Live Bird Markets in Nigeria, 2023-2024.Transboundary and emerging diseases · 2026Article
- Molecular Characterization of Publicly Available NDV F Gene Sequences From Bangladesh (2010-2024).Veterinary medicine international · 2026Article
- Whole genome sequence analysis and characterization of virulent Newcastle disease virus isolates from chicken and pheasants during 2020/21 outbreaks in Nepal.Virology journal · 2025Article
- Molecular characterization and genotyping of Newcastle disease viruses using whole-genome sequencing from poultry farms in parts of Amhara Regions, and central Ethiopia.Virology journal · 2025Article
- Field outbreak investigation and immunoinformatic analysis suggest potential immune evasion by Newcastle disease virus Sub-Genotype XIV.2 in Nigeria.Scientific reports · 2025Article
- Lentogenic avian paramyxovirus 1 of both class I and class II are circulating in danish wild and commercial waterfowl.Npj viruses · 2025Article
- Newcastle disease virus in Egyptian domestic poultry, 2019-2021: Molecular characterization, phylogenetic analysis, and coinfection with avian influenza A virus.Open veterinary journal · 2025Article
- Two Outbreaks of Pigeon Paramyxovirus 1 With High Mortality in Captive Pigeons (Transboundary and emerging diseases · 2025Article
- Enhanced Oncolytic Potential of Engineered Newcastle Disease Virus Lasota Strain through Modification of Its F Protein Cleavage Site.Microorganisms · 2024Article
- Review
- Molecular Identification and Phylogenetic Study Based on the Fusion Gene of Newcastle Disease Virus Isolated from Broiler Poultry Farms in Markazi Province, Iran.Archives of Razi Institute · 2023Article
- Identification of Embryonic Chicken Proteases Activating Newcastle Disease Virus and Their Roles in the Pathogenicity of Virus Used asJournal of virology · 2023Article
- Molecular characterization of Newcastle disease virus vaccines in Nigeria.Veterinary world · 2022Article
- Active Surveillance and Genetic Characterization of Prevalent Velogenic Newcastle Disease and Highly Pathogenic Avian Influenza H5N8 Viruses Among Migratory Wild Birds in Southern Egypt During 2015-2018.Food and environmental virology · 2022Article
- Article
- Root-preferential expression of Newcastle virus glycoproteins driven by NtREL1 promoter in tobacco hairy roots and evaluation of oral delivery in mice.Transgenic research · 2022Article
- Fusion Protein Cleavage Site Containing Three Basic Amino Acids Attenuates Newcastle Disease Virus in Chicken Embryos: Use as anFrontiers in microbiology · 2022Article
- Cellular CARD11 Inhibits the Fusogenic Activity of Newcastle Disease Virus via CBM Signalosome-Mediated Furin Reduction in Chicken Fibroblasts.Frontiers in microbiology · 2021Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Newcastle disease virus (NDV) is a contagious agent of Newcastle disease in avian species and seriously affects the poultry industry. The cleavage site of the viral F protein (Fcs) is a key determinant of membrane fusion and viral virulence. In this study, we investigated the precise effect of variable amino acid sequences of the Fcs on fusogenic activity. Based on viral pathogenicity, the Fcs sequences of natural isolates (n = 1572) are classified into eight types of virulent Fcs (VFcs) with the motif "G/R/K-R-Q/R/K-R/K-R↓F" and ten types of the avirulent Fcs (AFcs) with the motif "G/R/E-R/K/Q-Q-G/E-R↓L". The VFcs is only found in the Class II cluster of viral classification and not in Class I. The AFcs exists in both Class I and II isolates. The VFc and AFc types present an evolutionary relationship with temporal distribution and host species. Using a fusion assay in vitro, VFcs-1 "RRQKR↓F" and VFcs-2 "RRQRR↓F" show the highest efficiency in triggering membrane fusion. The neutral residue Q at the P3 position of the VFcs plays an enhancing role compared to effect of the basic residues R and K. A single residue K at P3 or P5 is less efficient of the fusogenic activity in the VFcs with all basic residues. Moreover, the cleavage efficiencies of F0 proteins with different types of Fcs motifs do not appear to affect membrane fusion. Our findings offer insight into the effect of amino acid variation of the Fcs on the fusion triggered by NDV.
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